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NFkB-p65 (phospho-S529) polyclonal antibody
SKU: orb644451
Description
Research Area
Cell Biology, Immunology & Inflammation, Signal Transduction
Images & Validation
−
| Tested Applications | IHC, IP, WB |
|---|---|
| Dilution Range | WB: 1:500~1:1000 IHC: 1:50~1:200 |
| Reactivity | Human |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Immunogen | Synthetic phosphopeptide derived from human NFkB-p65 around the phosphorylation site of Serine 529. |
| Molecular Weight | ~ 75 kDa |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE). |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH 7.2 |
| Concentration | 1mg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−p65; p65 NF kappaB; p65 NFkB; RELA; NFKB P65; Transcription Factor p65; Nuclear factor NF-kappa-B p65 subunit; NFkB-p65; NFKB3
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
IP
Immunoprecipitation
NFkB-p65 (phospho-S529) polyclonal antibody (orb644451)
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