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N/H/K-Ras rabbit pAb
SKU: orb765761
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Description
Images & Validation
−Item 1 of 4
| Tested Applications | ELISA, IHC-P, WB |
|---|---|
| Dilution range | Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. IF 1:100-300 Not yet tested in other applications. |
| Reactivity | Human, Mouse, Rat |
Key Properties
−| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Immunogen | The antiserum was produced against synthesized peptide derived from human RASH/RASK. AA range:1-50 |
| Molecular Weight | 21 |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | PBS with 0.02% sodium azide and 50% glycerol pH 7.4. |
| Concentration | 1 mg/ml |
| Disclaimer | For research use only |
Alternative Names
−Anti-NRAS antibody, anti-HRAS1 antibody, anti-GTPase NRas antibody, anti-Transforming protein N-Ras antibody, anti-HRAS antibody, anti-HRAS1 antibody, anti-GTPase HRas antibody, anti-H-Ras-1 antibody, anti-Ha-Ras antibody, anti-Transforming protein p21 antibody, anti-c-H-ras antibody, anti-p21ras antibody, anti-KRAS antibody, anti-KRAS2 antibody, anti-RASK2 antibody, anti-GTPase KRas antibody, anti-K-Ras 2 antibody, anti-Ki-Ras antibody, anti-c-K-ras antibody, anti-c antibody
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Immunofluorescence analysis of A549. 1, primary Antibody (red) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit IgG (H&L) - Alexa Fluor 594 Secondary antibody was diluted at 1:1000 (room temperature, 50min). 3, Picture B: DAPI (blue) 10min.
Western Blot analysis of various cells using N/H/K-Ras Polyclonal Antibody diluted at 1:1000.
Western Blot analysis of 293 cells using N/H/K-Ras Polyclonal Antibody diluted at 1:1000.
Immunohistochemistry analysis of paraffin-embedded human brain tissue, using RASH/RASK Antibody. The picture on the right is blocked with the synthesized peptide.
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
N/H/K-Ras rabbit pAb (orb765761)
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