NSE gamma antibody

SKU: orb2276526

Description

NSE gamma antibody validated for immunohistochemistry on 76 different Normal Tissues

Images & Validation

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Item 1 of 3

Tested Applications	IHC
Dilution Range	1:100-1:200
Reactivity	Human
Application Notes	Positive Control: In the colon, axons and ganglion cells in lamina propria and muscular wall must show at least a moderate NSE staining, while epithelial and lymphatic cells remain negative. Negative Control: In the colon, epithelial and lymphatic cells do not show NSE staining. Cellular Localization: Cytoplasmic Protocol Recommendations: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121°C in pH 7.8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37°C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer’s directions. Read more...

Key Properties

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Host	Mouse
Clonality	Monoclonal
Isotype	IgG1, kappa
Clone No.	MSVA-451M
Immunogen	A synthetic peptide of human NSE gamma (around aa416-433) (exact sequence is proprietary)
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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ENO2; ENOG; Enolase 2 gamma neuronal; Enolase2; Gamma-enolase; Neural enolase; Neuron specific gamma enolase; Neuron-specific enolase; NSE ; -phospho-D-glycerate hydrolyase

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A strong NSE immunostaining is seen in the granule cell layer and in the fibres of the white matter of the cerebellum.

Positive NSE immunostaining of islets of Langerhans.

Strong NSE immunostaining in an adrenal pheochromocytoma.

Quick Database Links

UniProt

P09104

UniProt Details

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No UniProt data available

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Protocol Information

IHC

Immunohistochemistry

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