p27 (phospho Thr198) rabbit pAb Antibody

SKU: orb767323

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Description

Rabbit polyclonal antibody to p27 (phospho-Thr198)

Images & Validation

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Item 1 of 3

Tested Applications	ELISA, WB
Dilution Range	Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications.
Reactivity	Human, Mouse, Rat

Key Properties

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Clonality	Polyclonal
Isotype	IgG
Immunogen	The antiserum was produced against synthesized peptide derived from human p27 Kip1 around the phosphorylation site of Thr198. AA range:149-198
Molecular Weight	27
Purification	The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
Concentration	1 mg/ml
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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Anti-CDKN1B antibody, anti-KIP1 antibody, anti-Cyclin-dependent kinase inhibitor 1B antibody, anti-Cyclin-dependent kinase inhibitor p27 antibody, anti-p27Kip1 antibody

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p27 (phospho Thr198) rabbit pAb Antibody

Western Blot analysis of VEC cells using Phospho-p27 (T198) Polyclonal Antibody.

Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using p27 Kip1 (Phospho-Thr198) Antibody.

Western blot analysis of lysates from HeLa cells, HepG2 cells and COLO cells, using p27 Kip1 (Phospho-Thr198) Antibody. The lane on the right is blocked with the phospho peptide.

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Protocol Information

Western Blot (IB, immunoblot)

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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