PBK/TOPK (Phospho-Thr9) Rabbit mAb [KD Validated] Antibody

SKU: orb1727670

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Description

Phosphorylates MAP kinase p38. Seems to be active only in mitosis. May also play a role in the activation of lymphoid cells. When phosphorylated, forms a complex with TP53, leading to TP53 destabilization and attenuation of G2/M checkpoint during doxorubicin-induced DNA damage.

Images & Validation

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Tested Applications	IHC, WB
Dilution Range	WB:1:500~1:2000IHC:1:50~1:200
Reactivity	Human

Key Properties

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Antibody Type	Recombinant Antibody
Host	Rabbit
Clonality	Monoclonal
Isotype	Rabbit IgG
Immunogen	A synthesized peptide derived from human Phospho-PBK/TOPK (Thr9)
Molecular Weight	40kDa
Purification	Affinity-chromatography
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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CT84; MAPKK like protein kinase; Nori3; PBK; PDZ binding kinas; Serine/threonine protein kinase; SPK; TOPK;

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PBK/TOPK (Phospho-Thr9) Rabbit mAb [KD Validated] Antibody

All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.

Western blot analysis of Phospho-PBK/TOPK (Thr9) expression in (1) HeLa cell lysate; (2) HeLa cell treated with Nocodazole lysate.

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UniProt

Q96KB5

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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