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Description
Research Area
Metabolism Research
Images & Validation
−Item 1 of 7
| Tested Applications | FC, IHC-P, WB |
|---|---|
| Dilution Range | WB - 1:2000, IHC-P - 1:25, FC - 1:25 |
| Reactivity | Human |
Key Properties
−| Host | Rabbit |
|---|---|
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Clone No. | BTD31352 |
| Immunogen | This PCSK9 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 153-183 amino acids from the N-terminal region of human PCSK9. Antigen Region: 153-183 aa. |
| Target | PCSK9 |
| Molecular Weight | 74286 Da |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
|---|---|
| Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Proprotein convertase subtilisin/kexin type 9, 3421-, Neural apoptosis-regulated convertase 1, NARC-1, Proprotein convertase 9, PC9, Subtilisin/kexin-like protease PC9, PCSK9, NARC1
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Immunohistochemical analysis of paraffin-embedded H. colorectal carcinoma section using PCSK9 Antibody (N-term). Diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.
Flow cytometric analysis of A431 cells using PCSK9 Antibody (N-term) (green) compared to an isotype control of rabbit IgG (blue). Diluted at 1:25 dilution. An Alexa Fluor 488 goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody.
PCSK9 Antibody (N-term) IHC analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the PCSK9 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Western blot analysis of PCSK9 Antibody (N-term) in Jurkat cell line lysates (35 ug/lane). PCSK9 (arrow) was detected using the purified Pab.
Western blot analysis of lysate from Hela cell line, using PCSK9 Antibody (N-term). Diluted at 1:1000. A goat anti-rabbit IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20 ug.
Western blot analysis of lysate from SW480 cell line, using PCSK9 Antibody (N-term). Diluted at 1:2000. A goat anti-rabbit IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20 ug.
Western blot analysis of lysate from SW480 cell line, using PCSK9 Antibody (N-term). Diluted at 1:1000. A goat anti-rabbit IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20 ug.
Quick Database Links
UniProt Details
− No UniProt data available
NCBI Reference Sequences
−Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product| Protein | NP_777596.2 |
|---|
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
FC
Flow Cytometry
PCSK9 Antibody (N-term) (orb1929351)
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