PDCD4 Antibody (Biotin)

SKU: orb344545

Description

Pdcd4 (phospho-S457) antibody (Biotin)

Images & Validation

−

Item 1 of 2

Tested Applications	ELISA, IP, WB
Dilution Range	ELISA: 1:20,000 - 1:100,000, IP: 1:1,000 - 1:5,000, WB: 1:2,000 - 1:10,000
Reactivity	Human, Mouse, Rat, Xenopus
Application Notes	Anti-Pdcd4 pS457 (MOUSE) Monoclonal Antibody Biotin Conjugated has been tested by ELISA and western blotting and is suitable for immunohistochemistry. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 62 kDa in size corresponding to phosphorylated Pdcd4 protein by western blotting in the appropriate cell lysate or extract. This phospho-specific monoclonal antibody reacts with human Pdcd4 pS457 and shows minimal reactivity by ELISA against the non-phosphorylated form of the immunizing peptide. Read more...

Key Properties

−

Antibody Type	Primary Antibody
Host	Mouse
Clonality	Monoclonal
Isotype	IgG1
Clone No.	9G6
Immunogen	This monoclonal antibody was produced by repeated immunizations with a synthetic peptide corresponding to residues surrounding Ser457 of the human Pdcd4 protein.
Target	PDCD4
Purity	Anti-Pdcd4 pS457 (MOUSE) Monoclonal Antibody Biotin Conjugated was purified from concentrated tissue culture supernate by Protein A chromatography. This antibody is specific for human Pdcd4 protein phosphorylated at Ser457. A BLAST analysis was used to suggest cross-reactivity with Pdcd4 from human, mouse, rat and Xenopus based on 100% homology with the immunizing sequence. Cross-reactivity with Pdcd4 from other sources has not been determined.
Conjugation	Biotin

Storage & Handling

−

Storage	Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Form/Appearance	Lyophilized
Buffer/Preservatives	Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Concentration	0.5 mg/mL
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

−

mouse anti-Pdcd4 pS457 Antibody biotin conjugation, biotin conjugated mouse anti-Pdcd4 pS457 Antibody, Death up-regulated gene protein antibody, Dug antibody, H731 antibody, Ma3 antibody, Neoplastic transformation inhibitor antibody, Neoplastic transformation inhibitor protein antibody, Nuclear antigen H731 antibody

Phospho-PDCD4 (Ser67) Rabbit Polyclonal Antibody (Biotin) [orb501811]

Applications:	FC, ICC, IF, IHC-Fr, IHC-P
Predicted Reactivity:	Bovine, Canine, Equine, Gallus, Porcine, Rabbit, Sheep
Reactivity:	Human, Mouse, Rat
Species/Host:	Rabbit
Clonality:	Polyclonal
Conjugation:	Biotin

Sizes Available: 100 μl

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at support@biorbyt.com.

ELISA of Mouse anti-Pdcd4 phospho S457 Biotin Conjugated antibody. Antigen: BSA conjugates of Pdcd4 phospho S457 and Pdcd4 non-phospho S457. Coating amount: 0.1 µg per well. Primary antibody: Pdcd4 phospho S457 Biotin Conjugated antibody at 5 µg/ml. Dilution series: 3-fold. Mid-point concentration: 5 ng/mL Pdcd4 phospho S457 Biotin Conjugated antibody. Secondary antibody: Peroxidase streptavidin secondary antibody at 1:10000. Substrate: TMB.

Western Blot of Mouse anti-Pdcd4 phospho S457 Biotin Conjugated antibody. Lane 1: Pdcd4 recombinant protein. Lane 2: none. Load: 100 ng per lane. Primary antibody: Pdcd4 phospho S457 Biotin Conjugated antibody at 1:1000 for overnight at 4°C. Secondary antibody: HRP Streptavidin secondary antibody at 1:40000 for 30 min at RT. Block: orb348637 for 30 min at RT. Predicted/Observed size: 50 kDa, 50 kDa for Pdcd4 phospho S457. Other band(s): Pdcd4 phospho S457 splice variants and isoforms

Quick Database Links

Gene Symbol

PDCD4

UniProt

Q53EL6

RefSeq:21735596

UniProt Details

−

No UniProt data available

NCBI Reference Sequences

−

Associated Accession Numbers

Curated reference sequences for the gene transcript and protein product

RefSeq	21735596

Documents Download

Datasheet

Product Information

Download

Request a Document

Protocol Information

Western Blot (IB, immunoblot)

View Protocol

ELISA

Enzyme-linked Immunosorbent Assay (EIA)

View Protocol

Immunoprecipitation

View Protocol

Find More Protocols