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PDCD4 Rabbit Polyclonal Antibody
SKU: orb704257
Featured
Description
Research Area
Apoptosis, Cell Cycle
Images & Validation
−Item 1 of 4
| Tested Applications | ELISA, IF, IHC-Fr, IHC-P, WB |
|---|---|
| Dilution Range | WB=1:500-2000, IHC-P=1:400-800, IHC-F=1:400-800, IF=1:100-500, ELISA=1:5000-10000 |
| Reactivity | Human |
| Predicted Reactivity | Mouse |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Recombinant human PDCD4 (1-260/469aa) |
| Target | PDCD4 |
| Molecular Weight | 51 kDa |
| Purification | Affinity purified by Protein A |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−PDCD-4; PDCD 4; Death up-regulated gene protein; Dug; H731; Ma3; MGC33046; MGC33047; Neoplastic transformation inhibitor; Neoplastic transformation inhibitor protein; Nuclear antigen H731; Nuclear antigen H731 like; Nuclear antigen H731 like protein; PDCD 4; Programmed cell death 4; programmed cell death 4(neoplastic transformation inhibitor); Programmed cell death protein 4; Protein 197/15a; Protein MA-3; RP11 348N5.4; Tis; Nuclear antigen H731-like; Pdcd4; PDCD4_HUMAN; Topoisomerase-inhibitor suppressed protein; Topoisomerase-inhibitor suppressed protein(programmed cell death 4).
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Paraformaldehyde-fixed, paraffin embedded (human placenta), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (PDCD4) Polyclonal Antibody, Unconjugated (orb704257) at 1:25 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human thyroid cancer), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (PDCD4) Polyclonal Antibody, Unconjugated (orb704257) at 1:25 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Sample: Lane 1: Hela (Human) Cell Lysate at 25 ug, Lane 2: PDCD4 knockout (KO) Hela (Human) Cell Lysate at 25 ug, Primary: Anti-PDCD4 (orb704257) at 1/1000 dilution, Secondary: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution, Predicted band size: 60 kD, Observed band size: 60 kD.
Sample: Lane 1: Hela (Human) Cell Lysate at 30 ug, Lane 2: 293T (Human) Cell Lysate at 30 ug, Lane 3: MCF-7 (Human) Cell Lysate at 30 ug, Lane 4: HepG2 (Human) Cell Lysate at 30 ug, Primary: Anti-PDCD4 (orb704257) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 60 kD, Observed band size: 58 kD.
Quick Database Links
Gene Symbol
PDCD4
UniProt
UniProt Details
− No UniProt data available
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Datasheet
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
IHC-Fr
Immunohistochemistry Frozen
IF
Immunofluorescence
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
PDCD4 Rabbit Polyclonal Antibody (orb704257)
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