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Description
Research Area
Cell Biology
Images & Validation
−Item 1 of 3
| Tested Applications | FC, IHC-P, WB |
|---|---|
| Dilution Range | WB: 1:1000, IHC-P: 1:10~50, FC: 1:10~50 |
| Reactivity | Human, Mouse, Rat |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Immunogen | This PDK2 antibody is generated from rabbits immunized with recombinant protein of human PDK2. Antigen Region: 16-307 aa. |
| Target | PDK2 |
| Molecular Weight | 46154 Da |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
|---|---|
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−[Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 2, mitochondrial, Pyruvate dehydrogenase kinase isoform 2, PDH kinase 2, PDKII, PDK2, PDHK2
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PDK2 Antibody flow cytometric analysis of Jurkat cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
PDK2 Antibody IHC analysis in formalin fixed and paraffin embedded skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the PDK2 Antibody for immunohistochemistry. Clinical relevance has not been evaluated.
All lanes: Anti-PDK2 Antibody at 1:1000 dilution. Lane 1: mouse heart lysates. Lane 2: rat heart lysates. Lane 3: human heart lysates. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 46 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
Quick Database Links
Gene Symbol
PDK2
UniProt
UniProt Details
− No UniProt data available
Documents Download
Datasheet
Product Information
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
FC
Flow Cytometry
PDK2 Antibody (orb1788014)
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