PELI3 Polyclonal Antibody

SKU: orb1420732

Description

Rabbit polyclonal antibody to PELI3.

Research Area

Epigenetics

Images & Validation

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Item 1 of 2

Tested Applications	ELISA, ICC, IF, IHC-Fr, IHC-P, WB
Reactivity	Porcine, Rat

Key Properties

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Host	Rabbit
Clonality	Polyclonal
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Disclaimer	For research use only

Alternative Names

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E3 ubiquitin-protein ligase pellino homolog 3, Pellino-3, 2.3.2.27, RING-type E3 ubiquitin transferase pellino homolog 3, PELI3

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Sample: Cerebrum (Mouse) Lysate at 40 ug. Heart (Mouse) Lysate at 40 ug. Primary: Anti-PELI3 at 1/300 dilution. Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution. Predicted band size: 51 kD. Observed band size: 51 kD.

Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (PELI3) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.

Quick Database Links

UniProt

Q8N2H9

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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IHC-P

Immunohistochemistry Paraffin

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IHC-Fr

Immunohistochemistry Frozen

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Immunofluorescence

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ICC

Immunocytochemistry

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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