Phospho ERK1+2 (Thr202 Rabbit Polyclonal Antibody

SKU: orb1294320

Description

Rabbit polyclonal antibody to ERK1+ ERK 2 (phospho-Thr202)

Images & Validation

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Item 1 of 2

Tested Applications	WB
Dilution Range	Western Blot 1:500-1:1000, Immunofluorescence 1:200-1:500
Reactivity	Human, Mouse, Rat

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	Rabbit-IgG
Immunogen	Synthetic peptide corresponding to residues surrounding Thr202 of human ERK
Target	Phospho ERK1+2 (Thr202/Tyr204)
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	100mM Tris Glycine, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

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Applications:	FC, ICC, IF, IHC-Fr, IHC-P
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Sizes Available: 50 μl, 100 μl, 200 μl

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Phospho-ERK1 (Thr202/Tyr204) + ERK2 (Thr183/Tyr185) Rabbit Polyclonal Antibody [orb783430]
Applications:
FC, IF, IHC
Predicted Reactivity:
Bovine, Canine, Equine, Gallus, Guinea pig, Porcine, Rabbit
Reactivity:
Human, Mouse, Rat
Species/Host:
Rabbit
Clonality:
Polyclonal
Conjugation:
Unconjugated
Sizes Available: 50 μl, 100 μl, 200 μl
Phospho-ERK1/2 (Thr202 + Tyr204) Rabbit Polyclonal Antibody (PE) [orb499483]
Applications:
FC, ICC, IF
Predicted Reactivity:
Bovine, Canine, Equine, Gallus, Guinea pig, Porcine, Rabbit
Reactivity:
Human, Mouse, Rat
Species/Host:
Rabbit
Clonality:
Polyclonal
Conjugation:
PE
Sizes Available: 100 μl
Phospho-ERK1/2 (Thr202 + Tyr204) Rabbit Polyclonal Antibody (FITC) [orb8026]
Applications:
FC, ICC, IF
Predicted Reactivity:
Bovine, Canine, Equine, Gallus, Guinea pig, Porcine, Rabbit
Reactivity:
Human, Mouse, Rat
Species/Host:
Rabbit
Clonality:
Polyclonal
Conjugation:
FITC
Sizes Available: 100 μl
Phospho-ERK1/2 (Thr202 + Tyr204) Rabbit Polyclonal Antibody (HRP) [orb483214]
Applications:
IHC-Fr, IHC-P
Predicted Reactivity:
Bovine, Canine, Equine, Gallus, Guinea pig, Porcine, Rabbit
Reactivity:
Human, Mouse, Rat
Species/Host:
Rabbit
Clonality:
Polyclonal
Conjugation:
HRP
Sizes Available: 100 μl

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Phospho ERK1+2 (Thr202 Rabbit Polyclonal Antibody

All lanes: Anti-ERK1+ERK2 phospho (Thr202 / Tyr204) antibody at 1/800 dilution. Lysates at 40 µg per lane. This blot was produced using a 10% SDS-PAGE. Nitrocellulose membrane was then blocked for an hour before being incubated with orb1294320 overnight at 4°C.

Specificity and sensitivity of Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) Antibody (orb1294320).

Quick Database Links

Gene Symbol

Phospho ERK1+2 (Thr202/Tyr204)

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Protocol Information

Western Blot (IB, immunoblot)

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