Phospho-MARCKS (Ser167/170) (C9) rabbit mAb APC conjugate Antibody

SKU: orb1946009

Description

MARCKS (myristoylated alanine-rich C kinase substrate) is a major PKC substrate expressed in all eukaryotic cells(1,2). It binds to and cross-links actin filaments to serve as a bridge between Ca2+/calmodulin and PKC signaling and attenuates phosphatidylinositol 4,5-bisphosphate plasma membrane signaling (3). MARCKS is involved with cell mobility, phagocytosis, membrane traffic, cell adhesion, and mitogenesis. Ser159, 163, 167 and 170 of MARCKS are phosphorylated by PKC in response to cell groeth and cellular stress (4). MARCKs phosphorylation is believe to induce its tranlocation from plasma membrane to cytoplasm.

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Tested Applications	FC
Dilution Range	For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells or 5 µL per 100 µL of staining volume. It is recommended that the reagent be titrated for optimal performance for each application. See product image legends for additional information.
Reactivity	Human, Mouse, Rat

Key Properties

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Host	Rabbit
Clonality	Monoclonal
Isotype	Rabbit IgG1k
Clone No.	MARCKSS167170-C9
Immunogen	A synthetic phospho-peptide corresponding to residues surrounding Ser167/170 of human phospho MARCKS
Purification	Protein A+G
Conjugation	APC

Storage & Handling

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Storage	2-8°C
Form/Appearance	Liquid
Buffer/Preservatives	1X PBS, 0.09% NaN3, 0.2% rAlbumin
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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Myristoylated alanine-rich C-kinase substrate, Protein kinase C substrate, PKCSL, 80 kDa protein light chain, MACS, PRKCSL

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Phospho-MARCKS (Ser167/170) (C9) rabbit mAb APC conjugate Antibody

Flow cytometric analysis of C6 cells, isotope IgG-FITC stained staurosprine treated cells as negative control (blue) or treated with staurosporine (red) or with UV + TPA (green) and stained using Phospho-MARCKS (Ser167/170) antibody MARCKSS167170-C9 APC conjugate. Cat. #orb1946009.

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UniProt

29966

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Protocol Information

Flow Cytometry

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