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Phospho-p53(T18) Antibody
SKU: orb1931240
Description
Research Area
Cancer Biology, Cell Biology, Infectious Disease & Virology, Microbiology, Neuroscience, Signal Transduction
Images & Validation
−Item 1 of 3
| Tested Applications | DOT, IHC-P, WB |
|---|---|
| Dilution Range | DB - 1:500, IHC-P - 1:100-500 |
| Reactivity | Human, Mouse |
| Predicted Reactivity | Monkey, Porcine, Rabbit |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
|---|---|
| Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Quality Guarantee
Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at support@biorbyt.com.
Dot blot analysis of anti-hp53-T18 Phospho-specific Pab on nitrocellulose membrane. 50ng of nonphospho-peptide or phospho-peptide were adsorbed on their respective dots. Antibody working concentration was 0.5ug per ml.
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Western blot analysis of anti-Phospho-p53-T18 Pab in, from left to right, A2058, Ramos, mouse lung, mouse testis, and HL60 cell line lysates. Phospho-p53-T18 (arrow) was detected using the purified Pab.
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UniProt
UniProt Details
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
DOT
Dot Blot
Phospho-p53(T18) Antibody (orb1931240)
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