Phospho-PKCa (Thr497) (F1) rabbit mAb Antibody

SKU: orb1946091

Description

PKCŒ± is a calcium-dependent isozyme of the PKC family that phosphorylates serine/threonine residues in apoptosis and cellular proliferation and differentiation pathways, including the MAPK cascade. PKCŒ± directly phosphorylated Raf-1, inducing survival genes. An increase in PKCŒ± is associated with multi-drug resistance in cancer cell lines, and increased expression in breast cancers is noted as causing a particularly malignant phenotype. Thus PKCŒ± has been the target of novel cancer therapeutics, with some promising developments in microRNA inhibitors. PKCŒ± is itself phosphorylated by mTOR. PKCŒ± also plays an important role in water regulator and solute absorption in the cell, where it regulates aquaporin 2 by initiating AQP2 ubiquination and lysosomal degradation.

Images & Validation

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Item 1 of 5

Tested Applications	FC, WB
Dilution Range	1µg/mL - 0.001µg/mL. It is recommended that the reagent be titrated for optimal performance for each application. See product image legends for additional information.
Reactivity	Human, Mouse, Rat

Key Properties

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Host	Rabbit
Clonality	Monoclonal
Isotype	Rabbit IgG1k
Clone No.	PKCaT497-F1
Immunogen	A synthetic phospho-peptide corresponding to residues surrounding Thr497 of human phospho PKCŒ±
Purification	Protein A+G
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	1X PBS, 0.02% NaN3, 50% Glycerol, 0.1% rAlbumin
Concentration	0.5 mg/mL
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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Protein kinase C alpha type, PKC-alpha, PRKCA, PKCA, PRKACA

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Phospho-PKCa (Thr497) (F1) rabbit mAb Antibody

Flow cytometric analysis of K562 cells secondary antibody only negative control (blue) or untreated (red) or treated with EGF + pervanadate (green) using PKCα (T497) antibody PKCaT497-F1 at 0.1 μg/mL. Cat. #orb1946091.

Western blot analysis of HT1080 cell extract, untreated or treated with TPA using 0.05 ug/mL Phospho-PKCa (Thr497) antibody AWBPKCAT497-F1. Cat. #orb1946091

Western blot analysis of HT1080 cell extract untreated or treated with TPA using 0.05 μg/mL Phospho-PKCα (Thr497) antibody PKCaT497-F1 Cat. #orb1946091 or Company A antibody at 1 μg/mL (manufacturer's recommended concentration) developed using the same exposure.

Flow cytometric analysis of 3T3 cells, secondary antibody only negative control (blue) or treated with imatinib (grey) or with pervanadate (orange) using 0.1 μg/mL isotype control Cat. #orb1946241, or imatinib (red) or pervanadate (green) using PKCα (T497) antibody PKCaT497-F1 at 0.1μg/mL Cat. #orb1946091.

Western blot analysis of C6 cell extract treated with Anisomycin using 0.1 μg/mL PKCα (T497) antibody PKCaT497-F1. Cat. #orb1946091.

Quick Database Links

UniProt

P17252

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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Flow Cytometry

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