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PKM Antibody
Description
Research Area
Images & Validation
−
| Tested Applications | ELISA, IF, WB |
|---|---|
| Dilution Range | WB: 1:500-1:1000, IF: 1:100-1:200 |
| Reactivity | Human |
Key Properties
−| Host | Rabbit |
|---|---|
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Peptide sequence around phosphorylation site of serine 37(I-D-S(p)-P-P) derived from Human PKM2. |
| Target | PKM |
| Purification | Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | liquid |
| Buffer/Preservatives | Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
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Protocol Information
PKM Antibody (orb572160)
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