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Phospho-Rb(S811) Antibody
SKU: orb1931227
Description
Research Area
Cancer Biology, Cell Biology, Infectious Disease & Virology
Images & Validation
−Item 1 of 3
| Tested Applications | DOT, IF, IHC-P |
|---|---|
| Dilution Range | DB - 1:500, IHC-P - 1:100-500, IF - 1:10-50 |
| Reactivity | Human |
| Predicted Reactivity | Mouse, Rat |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Molecular Weight | 106159 Da |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
|---|---|
| Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Similar Products
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Phospho-RB-S811 polyclonal antibody [orb1786941]
WB
Human
Rabbit
Polyclonal
Unconjugated
50 μl, 100 μl, 200 μl
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Dot blot analysis of anti-hRb-S811 Phospho-specific Pab on nitrocellulose membrane. 50ng of nonphospho-peptide or phospho-peptide were adsorbed on their respective dots. Antibody working concentration was 0.5ug per ml.
Confocal immunofluorescent analysis of Phospho-Rb-S811 Antibody with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
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Protocol Information
IHC-P
Immunohistochemistry Paraffin
IF
Immunofluorescence
DOT
Dot Blot
Phospho-Rb(S811) Antibody (orb1931227)
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