Phospho-Ship2 (Tyr1135) (1D2) rabbit mAb Antibody

SKU: orb1946183

Description

Ship2 (SH2-containing inositol phosphatase 2, phospho Ship2) is a homolog of Ship1. Ship2 is highly expressed in the heart, in skeletal muscle, and in the placenta (1). SHIP2 negatively regulates insulin signaling (2), and Ship2 polymorphisms have been linked to hyperglycemia (3). Phospho Ship2 has been identified as a potential therapeutic target for the treatment of obesity and type 2 diabetes (4,5). The Ship2 residue Tyr1135 is phosphorylated in human cancer cells (6-8).

Research Area

Signal Transduction

Images & Validation

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Item 1 of 3

Tested Applications	FC
Dilution Range	1µg/mL - 0.001µg/mL. It is recommended that the reagent be titrated for optimal performance for each application. See product image legends for additional information.
Reactivity	Human, Mouse

Key Properties

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Host	Rabbit
Clonality	Monoclonal
Isotype	Rabbit IgG1k
Clone No.	Ship2Y1135-1D2
Immunogen	A synthetic phospho-peptide corresponding to residues surrounding Tyr1135 of human phospho Ship2
Purification	Protein A+G
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	1X PBS, 0.02% NaN3, 50% Glycerol, 0.1% rAlbumin
Concentration	0.5 mg/mL
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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Phosphatidylinositol 3,4,5-trisphosphate 5-phosphatase 2, Inositol polyphosphate phosphatase-like protein 1, INPPL-1, Protein 51C, SH2 domain-containing inositol 5'-phosphatase 2

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Phospho-Ship2 (Tyr1135) (1D2) rabbit mAb Antibody

Peptide blocking flow cytometric analysis of U937 cells secondary antibody only negative control (light blue) or untreated (red) or treated with IFNα IL-4 and pervanadate (green) or untreated and blocked with phospho-peptide (black) or treated and blocked with phospho peptide (gold) or untreated and blocked with non-phospho peptide (dark blue) or treated and blocked with non-phospho peptide (purple) using Phospho-Ship2 (Tyr1135) antibody Ship2Y1135-1D2 at 0.5μg/mL. Cat. #orb1946183.

Flow cytometric analysis of C2C12 cells secondary antibody only negative control (blue) or 1 μg/mL of isotype control Cat. #orb1946241 (orange) or untreated (red) or treated with staurosporine (green) using Phospho-Ship2 (Tyr1135) antibody Ship2Y1135-1D2 at 1 μg/mL. Cat. #orb1946183.

Flow cytometric analysis of U937 cells secondary antibody only negative control (blue) or untreated (red) or treated with IFNα IL-4 and pervanadate (green) using Phospho-Ship2 (Tyr1135) antibody Ship2Y1135-1D2 at 0.5μg/mL. Cat. #orb1946183.

Quick Database Links

UniProt

O15357

UniProt Details

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No UniProt data available

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Protocol Information

Flow Cytometry

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