Phospho-Smad2 (Ser255) Recombinant Rabbit Monoclonal Antibody

SKU: orb559124

Description

Research Area

Cell Biology, Cell Signaling, Cytoplasmic, Intracellular Signaling Proteins

Images & Validation

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Item 1 of 1

Tested Applications	WB
Dilution Range	WB=1:500-1000
Reactivity	Human
Predicted Reactivity	Mouse

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Recombinant
Isotype	IgG
Clone No.	8C12
Immunogen	KLH conjugated Synthesised phosphopeptide derived from human Smad2 around the phosphorylation site of Ser255 TL(p-S)PV
Target	SMAD2
Molecular Weight	58 kDa
Purification	Affinity purified by Protein A
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Concentration	1mg/ml
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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Smad2(S255); Smad2(phospho S255); phospho-Smad2(Ser255); p-Smad2(Ser255); phospho-Smad2(S255); Mothers against decapentaplegic homolog 2; SMAD 2; Mothers against DPP homolog 2; Smad2; hMAD 2; hSMAD2; JV18 1; JV18; JV181; MAD; MAD Related Protein 2; MADH2; MADR2; MGC22139; MGC34440; Mothers Against Decapentaplegic Homolog 2; mothers against DPP homolog 2; SMAD 2; SMAD; SMAD2; SMAD2_HUMAN.

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Phospho-Smad2 (Ser255) Recombinant Rabbit Monoclonal Antibody

Blocking buffer: 5% NFDM/TBST, Primary Ab, dilution: 1:1000, Primary Ab incubation condition: 2 hours at room temperature, Secondary Ab: Goat Anti-Rabbit IgG H&L (HRP), Lysate: 1: (-) HeLa, 2: (+) HeLa + OA (100 mM, 60 min) + CA (100 mM, 60 min), Protein loading quantity: 20 µg, Exposure time: 60 s, Predicted MW: 52 kDa, Observed MW: 58 kDa.

Quick Database Links

Gene Symbol

SMAD2

UniProt

Q15796

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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