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Phospho-Tau (Ser416) Rabbit Polyclonal Antibody
Description
Images & Validation
−| Tested Applications | FC, WB |
|---|---|
| Dilution range | WB=1:500-2000, Flow-Cyt=1μg/Test |
| Reactivity | Human, Mouse, Rat |
| Predicted Reactivity | Bovine, Canine, Equine, Gallus |
Related Conjugates & Formulations
−Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | KLH conjugated Synthesised phosphopeptide derived from human Tau around the phosphorylation site of Ser416 TG(p-S)ID |
| Target | MAPT |
| Molecular Weight | 52/79 kDa |
| Purification | Affinity purified by Protein A |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Disclaimer | For research use only |
Alternative Names
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Blank control (blue line): MCF 7 (fixed with 70% methanol (Overnight at 4°C) and then permeabilized with 90% ice-cold methanol for 30 min on ice). Primary Antibody (green line): Rabbit Anti-phospho-Tau (Ser416) antibody (orb7069), dilution: 1 µg/10^5 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC, dilution: 1 µg/Test.

Sample: Cerebrum (Mouse) Lysate at 40 ug, Cerebrum (Rat) Lysate at 40 ug, Primary: Anti-phospho-Tau (Ser416) (orb7069) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 52/79 kD, Observed band size: 52 kD.

Sample: Lane 1: Cerebrum (Rat) Lysate at 40 ug, Lane 2: Cerebrum (Mouse) Lysate at 40 ug, Lane 3: Cerebellum (Rat) Lysate at 40 ug, Lane 4: Cerebellum (Mouse) Lysate at 40 ug, Primary: Anti-phospho-Tau (Ser416) (orb7069) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 50-70 kD, Observed band size: 55'62kD.

Sample: Lane 1: Cerebrum (Rat) Lysate at 40 ug, Lane 2: Cerebrum (Mouse) Lysate at 40 ug, Lane 3: Cerebellum (Rat) Lysate at 40 ug, Lane 4: Cerebellum (Mouse) Lysate at 40 ug, Primary: Anti-phospho-Tau (Ser416) (orb7069) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 50-70 kD, Observed band size: 55'62kD.
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Phospho-Tau (Ser416) Rabbit Polyclonal Antibody (orb7069)
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