Phosphoserine Antibody (Biotin)

SKU: orb396885

Description

Rabbit polyclonal antibody against Phosphoserine conjugated to Biotin.

Research Area

Protein Biochemistry, Signal Transduction

Images & Validation

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Tested Applications	ELISA, ICC, IF, IHC, IP, WB
Dilution Range	WB (1:500), ICC/IF (1:50), ELISA (1:250), IP (1:100)
Reactivity	All
Application Notes	2 µg/ml was sufficient for detection of phosphorylation signal in western blot analysis using human MMRU cells treated with 0.1 µM okadaic acid.

Key Properties

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Host	Rabbit
Clonality	Polyclonal
Immunogen	Phosphoserine conjugated to KLH, and phosvitin mixture
Target	Phosphoserine
Purification	Peptide Affinity Purified
Conjugation	Biotin

Storage & Handling

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Storage	Conjugated antibodies should be stored according to the product label
Buffer/Preservatives	136.36mM Ethanolamine, 133.23 mM Chlorides, 9.55mM Phosphates, 9.55mM Sodium Bicarbonate.
Concentration	0.25 mg/ml
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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O-phospho-L-serine, Phospho-serine, OPS, Phospho-ser, pS, pSer

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Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Phosphoserine Polyclonal Antibody. Tissue: Cervical cancer cell line (HeLa). Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-Phosphoserine Polyclonal Antibody at 1:50 for 12 hours at 4°C. Secondary Antibody: R-PE Goat Anti-Rabbit (yellow) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Cytoplasm. Nucleus. Magnification: 100x.

Western blot analysis of Mouse Spleen lysates showing detection of Phosphoserine protein using Rabbit Anti-Phosphoserine Polyclonal Antibody. Primary Antibody: Rabbit Anti-Phosphoserine Polyclonal Antibody at 1:1000. Bands are responsive to treatment with varying long UV wavelengths: A (0), B (50), C (200), D (400), and E (treated with 0.1μM okadaic acid).