Phosphoserine Antibody

SKU: orb420304

This product can be custom made

Description

Phosphoserine antibody

Images & Validation

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Item 1 of 1

Tested Applications	ELISA, IHC, IP, WB
Dilution Range	ELISA: 1:1,000-1:2,500, IP: 10 µg/mg protein sample, WB: 1:500-1:1,000
Reactivity	Mouse
Application Notes	Phosphoserine Antibody is tested for use in ELISA, western blotting, Immunohistochemistry, and immunoprecipitation assays. The antibody reacts specifically with phosphoserine and shows minimal reactivity by ELISA and western blotting with proteins containing phosphotyrosine or phosphothreonine residues. The antibody reacts with free phosphoserine, phosphoserine conjugated to carriers such as thyroglobulin or BSA, and detects the presence of phosphoserine in proteins of both unstimulated and stimulated cell lysates. Although not tested, this antibody is likely functional in RIA and flow cytometry. Read more...

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	Phosphoserine Antibody was prepared from whole rabbit serum produced by repeated immunizations with phosphoserine conjugated KLH.
Purity	Anti-Phosphoserine Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using phosphorylated serine peptide coupled to agarose. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit IgG. Reactivity is specific for phosphoserine and minimal cross-reactivity is observed against phosphotyrosine or phosphothreonine.
Conjugation	Unconjugated

Storage & Handling

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Storage	Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Form/Appearance	Liquid (sterile filtered)
Buffer/Preservatives	Preservative: None. Stabilizer: 50% (v/v) Glycerol; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Concentration	0.25 mg/mL
Expiration Date	12 months from date of receipt.
Dry Ice Shipping	Please note: This product requires shipment on dry ice. A dry ice surcharge will apply.
Disclaimer	For research use only

Alternative Names

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pS antibody, phospho serine AB, PhosphoSerine (pS) reactive antibody, proteinogenic amino acid antibodies, Phosphoserine [pS] polyclonal antibody, PhosphoSerine antibody produced in rabbit

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at support@biorbyt.com.

Biorbyt's Affinity Purified anti-phosphoserine (pS) antibody is shown to detect phosphorylated proteins present in UV-treated mouse spleen cell lysates (top panel).

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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Immunoprecipitation

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