Phosphoserine Antibody

SKU: orb613989

Description

Rabbit Anti-Phosphoserine Polyclonal

Research Area

Protein Biochemistry, Signal Transduction

Images & Validation

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Item 1 of 2

Tested Applications	ELISA, ICC, IF, IHC, IP, WB
Dilution Range	WB (1:500), ICC/IF (1:50), ELISA (1:250), IP (1:100)
Reactivity	All
Application Notes	2 µg/ml was sufficient for detection of phosphorylation signal in western blot analysis using human MMRU cells treated with 0.1 µM okadaic acid.

Key Properties

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Host	Rabbit
Clonality	Polyclonal
Immunogen	Phosphoserine conjugated to KLH, and phosvitin mixture
Target	Phosphoserine
Purification	Peptide Affinity Purified
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	PBS, 50% glycerol, 0.01% sodium azide Storage buffer changes when conjugated
Concentration	0.25 mg/ml
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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O-phospho-L-serine, Phospho-serine, OPS, Phospho-ser, pS, pSer

Quality Guarantee

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Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Phosphoserine Polyclonal Antibody. Tissue: Cervical cancer cell line (HeLa). Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-Phosphoserine Polyclonal Antibody at 1:50 for 12 hours at 4°C. Secondary Antibody: R-PE Goat Anti-Rabbit (yellow) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Cytoplasm. Nucleus. Magnification: 100x.

Western blot analysis of Mouse Spleen lysates showing detection of Phosphoserine protein using Rabbit Anti-Phosphoserine Polyclonal Antibody. Primary Antibody: Rabbit Anti-Phosphoserine Polyclonal Antibody at 1:1000. Bands are responsive to treatment with varying long UV wavelengths: A (0), B (50), C (200), D (400), and E (treated with 0.1μM okadaic acid).

Quick Database Links

Gene Symbol

Phosphoserine

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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Immunofluorescence

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ICC

Immunocytochemistry

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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Immunoprecipitation

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