Pifithrin-μ

SKU: orb1300977

Description

Pifithrin-μ (NSC-303580) is a small molecule inhibitor targeting p53 and HSP70. It exhibits neuroprotective properties and demonstrates antitumor activity, making it a useful research tool for studying apoptosis and cellular stress in both in vitro and in vivo experimental models.

Research Area

Cell Biology, Metabolism Research, Signal Transduction

Images & Validation

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Key Properties

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CAS Number	64984-31-2
MW	181.21
Purity	99.79%
Formula	C₈H₇NO₂S
SMILES	NS(=O)(=O)C#Cc1ccccc1
Target	p53,MDM-2/p53,HSP,Autophagy
Solubility	10% DMSO+40% PEG300+5% Tween 80+45% Saline:2 mg/mL (11.04 mM);Ethanol:13.1 mg/mL (72.29 mM);DMSO:250 mg/mL (1379.61 mM)

Bioactivity

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In Vivo	Pifithrin-μ (40 mg/kg, ip) provides protection against p53-dependent apoptosis in C57B1/6J mice exposed to 8 or 9 Gy of total body gamma radiation . Additionally, in a xenograft mouse model, Pifithrin-μ significantly enhances TRAIL-induced inhibition of MiaPaca-2 tumor growth . Read more...
In Vitro	Pifithrin-μ interferes with p53 binding to mitochondria and inhibits rapid p53-dependent apoptosis of primary cell cultures of mouse thymocytes in response to gamma radiation. Pifithrin-μ, as an inhibitor of inducible HSP70, significantly inhibited cell viability with IC50 values ranging from 2.5 to 12.7 μM in acute myeloid leukemia (AML) and acute lymphoblastic leukemia (ALL) cell lines, as well as in primary AML blasts. Pifithrin-μ is also highly active in primary AML blasts with a median IC50 of 8.9 μ M(range 5.7-37.2 μ M) and induces apoptosis and cell cycle arrest in a dose-dependent fashion. In addition, Pifithrin-μ also increases the active form of caspase-3 and reduces AKT and ERK1/2 in NALM-6 cells. Pifithrin-μ increases Annexin V(+) cells in both caspase-dependent and -independent manners and promotes TRAIL-induced apoptosis and arrested cancer cell growth. Read more...
Cell Research	The number of attached cells is estimated by staining with 0.5% methylene blue and measuring optical density using a Multiscan Ascent microplate reader. Cell viability is determined in suspension of short-term culture of primary thymocytes by staining them with 0.1% trypan blue2 or by analysis of annexin- or propidium iodide (PI)-positive cells by FACScan. (Only for Reference) Read more...

Storage & Handling

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Storage	store at low temperature \| Powder: -20°C for 3 years \| In solvent: -80°C for 1 year \| Shipping with blue ice/Shipping at ambient temperature.
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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Autophagy, 2-Phenylethynesulfonamide, NSC 303580, inhibit, NSC303580, NSC-303580, p53, MDM-2/p53, Heat shock proteins, Inhibitor, HSP, PFTμ, PFTmu, Pifithrinμ, Pifithrin-μ, Pifithrin μ, Pifithrin-mu

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Compound Identifiers

PubChem CID

327653 ↗

Key Properties

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No computed properties available.

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Pifithrin-μ (orb1300977)

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Purity:	>98% (HPLC)
CAS Number:	64984-31-2
MW:	181.21
Formula:	C₈H₇NO₂S

Pifithrin-μ

Description

Research Area

Images & Validation

Key Properties

Bioactivity

Storage & Handling

Alternative Names

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