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Pirfenidone

SKU: orb1305557

Description

Pirfenidone

Research Area

Immunology & Inflammation, Infectious Disease & Virology, Pharmacology & Drug Discovery, Stem Cell & Developmental Biology

Images & Validation

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Pirfenidone

Key Properties

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CAS Number53179-13-8
MW185.22
Purity99.80%
FormulaC12H11NO
SMILESO=C1N(C=C(C)C=C1)C2=CC=CC=C2
TargetTGF-beta/Smad,CCR
SolubilityH2O:25 mg/mL (134.97 mM);5% DMSO+95% Saline:1.86 mg/mL (10.04 mM);DMSO:237.5 mg/mL (1282.26 mM)

Bioactivity

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Target IC50
HFL1 cells:0.71 μM|NIH3T3 cells:454 μM|MRC5 cells:14.44 mM
In Vivo
METHODS: To examine the effect of Pirfenidone on pulmonary fibrosis, Pirfenidone (30-100 mg/kg, 0.5% carboxymethylcellulose) was administered orally three times a day for 42 days to ICR mice with bleomycin-induced pulmonary fibrosis. RESULTS: Pirfenidone inhibited pulmonary inflammatory edema and significantly suppressed pulmonary fibrosis. During the development of bleomycin-induced pulmonary fibrosis in mice, Pirfenidone exerted its antifibrotic effects by regulating the levels of lung IFN-γ, bFGF and TGF-β1.
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In Vitro
METHODS: Mouse macrophage-like cell line RAW264.7 was treated with lipopolysaccharide (1 µg/mL) and Pirfenidone (30-300 µg/mL) for 8 h. TNF-α was detected by ELISA assay. RESULTS: Exposure of RAW264.7 cells to Pirfenidone significantly inhibited cell-associated and secreted TNF-α levels. METHODS: Three stromal cell lines from control lungs and three cell lines derived from idiopathic pulmonary fibrosis (IPF) were treated with Pirfenidone (0.1-1 mM) for 6 days and cell viability was measured by MTT assay. RESULTS: Pirfenidone inhibited cell proliferation in a dose-dependent manner. on day 6 of 1 mM Pirfenidone treatment, proliferation was reduced to 47% for control cells and 42% for IPF cells.
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Cell Research
Pirfenidone (PFD) is dissolved in DMSO and stored, and then diluted with appropriate media before use. HLECs are seeded in 96-well plates (1×104 cells/well) for 24 hours in α-MEM/10% FBS/1%NEAA, and are cultured in stationary tubes in serum-free medium for 24 hours. And then the culture medium is removed and cells are bathed in α-MEM with 10% FBS and 1% NEAA supplemented with 0, 0.01, 0.1, 0.2, 0.3, 0.5, or 1 mg/mL Pirfenidone for 0, 4, 12, 24, 48, or 72 hours. After incubation with 180 μL α-MEM and 20 μL of 5 mg/mL MTT for 4 hours at 37°C, the MTT solution is discarded. The Formosan precipitates are dissolved in 180 μL DMSO by agitating the dishes for 10 minutes at 200 rpm on an orbital shaker. The absorbance at 490 nm in each well is read with a micro plate reader. We further examined the effects of PFD by refining the concentrations at 0.2, 0.25, 0.3, 0.4, 0.5 and 0.6 mg/mL using the MTT assay.
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Storage & Handling

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Storagekeep away from direct sunlight,keep away from moisture | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

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Pirfenidone, Smad, S 7701, S7701, S-7701, S-7701,AMR-69, TGF-b/Smad, TGFb, TGF-β/Smad, TGFβ, TGF-beta/Smad, TGFbeta, TGF-beta, TGFbeta/Smad, Transforming growth factor beta, TGFβ2, CC chemokine receptor, AMR 69, AMR69, AMR-69, CCR, inhibit, Inhibitor

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Quality Guarantee

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Compound Identifiers

PubChem CID
40632 ↗

Key Properties

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No computed properties available.

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% DMSO +
%+
% Tween 80 +
%

Available Sizes

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25 mg
$ 70.00
1 ml x 10 mM (in DMSO)
$ 80.00
50 mg
$ 80.00
100 mg
$ 100.00
200 mg
$ 120.00
500 mg
$ 140.00
1 g
$ 190.00
5 g
$ 260.00
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