Phospho-RNA polymerase II Antibody (pS5) / POLR2A

SKU: orb606982

Description

RNA polymerase II (Pol II) is an enzyme that is composed of 12 subunits and is responsible for the transcription of protein-coding genes. Transcription initiation requires Pol II-mediated recruitment of transcription machinery to a target promoter, thereby allowing transcription to begin. The largest subunit of Pol II (referred to as RPB1 or RPB205) is a 1,840 amino acid protein that contains one C2H2-type zinc finger and a C-terminal domain comprised of several heptapeptide repeats. Although Pol II function requires the cooperation of all twelve subunits, the largest subunit conveys Pol II catalytic activity and, together with the second largest subunit, forms the active center of the Pol II enzyme. Additionally, the large subunit participates in forming the DNA-binding domain of Pol II, a groove that is necessary for transcription of the DNA template. Without proper function of the large subunit, mRNA synthesis and subsequent transcription elongation cannot occur.

Research Area

Epigenetics, Infectious Diseases

Images & Validation

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Tested Applications	FACS, IF, IHC-P, WB
Dilution Range	Flow cytometry: 1-2ug/10^6 cells,Immunofluorescence: 1-2ug/ml,Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
Reactivity	Human
Application Notes	Optimal dilution of the recombinant phospho-RNA polymerase II antibody should be determined by the researcher.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Key Properties

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Antibody Type	Primary Antibody
Host	Mouse
Clonality	Monoclonal
Isotype	Mouse IgM, kappa
Clone No.	CTD 8A7
Immunogen	The immunogen for this phospho-RNA polymerase II antibody was 10 repeats of synthetic peptide YSPTSPS using chemically synthesized phospho-S5.
Purification	PEG precipitation
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

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Phospho-RNA polymerase II Antibody (pS5) / POLR2A

Western blot testing of human HepG2, K562, and SK-BR-3 cell lysate with phospho-RNA polymerase II antibody (clone CTD 8A7). Routinely observed molecular weight: 200-250 kDa.

IHC staining of FFPE human colon carcinoma with phospho-RNA polymerase II antibody (clone CTD 8A7). HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.