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Rabbit ALDH18A1/P5CS Antibody
SKU: orb1522299
Description
Research Area
Metabolism Research, Neuroscience
Images & Validation
−Item 1 of 3
| Tested Applications | IP, WB |
|---|---|
| Dilution Range | WB - 1:10,000 - 1:25,000; IP - 2 - 10 µg/mg lysate |
| Reactivity | Human, Mouse |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Between 575 and 625 |
| Target | ALDH18A1/P5CS |
| Purification | Antigen Affinity Purified |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | 2 - 8°C |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide |
| Concentration | 1000 µg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−ADCL3; aldehyde dehydrogenase family 18 member A1; ARCL3A; delta1-pyrroline-5-carboxlate synthetase; delta-1-pyrroline-5-carboxylate synthase; delta-1-pyrroline-5-carboxylate synthetase; Gamma-glutamyl kinase; Gamma-glutamyl phosphate reductase (GPR); Glutamate 5-kinase (GK); Glutamate-5-semialdehyde dehydrogenase; Glutamyl-gamma-semialdehyde dehydrogenase; GSAS; P5CS; PYCS; pyrroline-5-carboxylate synthetase (glutamate gamma-semialdehyde synthetase); spastic paraplegia 9 (autosomal dominant); Spastic paraplegia-9 (spastic paraparesis with amyotrophy, cataracts and gastroesophageal reflux); SPG9; SPG9A; SPG9B
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Detection of human ALDH18A1/P5CS by western blot. Samples: Whole cell lysate (10 µg) from Hep-G2, MCF-7, HeLa, and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-ALDH18A1/P5CS antibody orb1522299 used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Detection of mouse ALDH18A1/P5CS by western blot. Samples: Whole cell lysate (10 µg) from NIH 3T3, CT26, CH27, and TCMK-1 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-ALDH18A1/P5CS antibody orb1522299 used for WB at 0.04 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Detection of human ALDH18A1/P5CS by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-ALDH18A1/P5CS antibody orb1522299 used for IP at 6 µg per reaction.
Quick Database Links
UniProt Details
− No UniProt data available
NCBI Reference Sequences
−Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product| Protein | NP_002851.2 |
|---|
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Protocol Information
WB
Western Blot (IB, immunoblot)
IP
Immunoprecipitation
Rabbit ALDH18A1/P5CS Antibody (orb1522299)
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