Rabbit CCT3 Antibody

SKU: orb1524799

Description

CCT3 Antibody

Research Area

Metabolism Research

Images & Validation

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Item 1 of 3

Tested Applications	IHC, IP, WB
Dilution Range	WB - 1:2,000 - 1:10,000; IP - 2 - 10 µg/mg lysate; IHC - 1:100 - 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Reactivity	Human, Mouse
Predicted Reactivity	Rat
Application Notes	Format: Whole IgG

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	Between 325 and 375
Target	CCT3
Purification	Antigen Affinity Purified
Conjugation	Unconjugated

Storage & Handling

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Storage	2 - 8°C
Form/Appearance	Liquid
Buffer/Preservatives	Tris-buffered Saline containing 0.1% rAlbumin and 0.09% Sodium Azide
Concentration	200 µg/ml
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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CCTG; CCT-gamma; chaperonin containing TCP1, subunit 3 (gamma); hTRiC5; PIG48; T-complex protein 1 subunit gamma; T-complex protein 1, gamma subunit; TCP1 (t-complex-1) ring complex, polypeptide 5; TCP-1-gamma; TRIC5

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Detection of human and mouse CCT3 by western blot (h and m) and immunoprecipitation (h). Samples: Whole cell lysate from HeLa (15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) , HEK293T (T; 50 µg) , Jurkat (J; 50 µg) and mouse NIH 3T3 (M; 50 µg) cells. Antibodies: Affinity purified rabbit anti-CCT3 antibody orb1524799 used for WB at 0.04 µg/ml (A) and 0.4 µg/ml (B) and used for IP at 6 µg/mg lysate. CCT3 was also immunoprecipitated by rabbit anti-CCT3 antibody, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 1 second (A and B).

Detection of mouse CCT3 by immunohistochemistry.Sample: FFPE section of mouse teratoma. Antibody: Affinity purified rabbit anti-CCT3 (Cat. No. orb1524799) used at a dilution of 1: 200 (1µg/ml). Detection: DAB

Detection of human CCT3 by immunohistochemistry.Sample: FFPE section of human lung carcinoma. Antibody: Affinity purified rabbit anti-CCT3 (Cat. No. orb1524799) used at a dilution of 1: 200 (1µg/ml). Detection: DAB. Counterstain: hematoxylin (blue).

Quick Database Links

Gene Symbol

CCT3

UniProt

P49368

RefSeq (Protein):NP_005989.3

UniProt Details

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No UniProt data available

NCBI Reference Sequences

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Associated Accession Numbers

Curated reference sequences for the gene transcript and protein product

Protein	NP_005989.3

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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Immunoprecipitation

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