Rabbit CHD8 Antibody

SKU: orb1528802

Description

CHD8 Antibody

Images & Validation

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Item 1 of 4

Tested Applications	IHC, IP, WB
Dilution Range	WB - 1:2,000 - 1:10,000; IP - 2 - 10 µg/mg lysate; IHC - 1:200 to 1:1000. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
Reactivity	Human, Mouse
Application Notes	Format: Whole IgG

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	Between 2531 and C-terminus
Target	CHD8
Purification	Antigen Affinity Purified
Conjugation	Unconjugated

Storage & Handling

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Storage	2 - 8°C
Form/Appearance	Liquid
Buffer/Preservatives	Tris-buffered Saline containing 0.1% rAlbumin and 0.09% Sodium Azide
Concentration	200 µg/ml
Disclaimer	For research use only

Alternative Names

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ATP-dependent helicase CHD8; AUTS18; axis duplication inhibitor; CHD-8; chromodomain-helicase-DNA-binding protein 8; duplin; helicase with SNF2 domain 1; HELSNF1

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Detection of human and mouse CHD8 by western blot.Samples: Whole cell lysate from HEK293T, K-562, MCF-7, and NIH 3T3 cells. Antibody: Affinity purified Rabbit anti-CHD8 antibody orb1528802 at 0.1 µg/ml.

Detection of human CHD8 by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-CHD8 used at a dilution of 1:1000 (0.2µg/ml).

Detection of mouse CHD8 by immunohistochemistry. Sample: FFPE section of mouse CT26 colon carcinoma. Antibody: Affinity purified rabbit anti-CHD8 used at a dilution of 1:1000 (0.2µg/ml).

Detection of human CHD8 by western blot of immunoprecipitates.Samples: Whole cell lysate from HEK293T cells. Antibodies: Rabbit anti-CHD8 antibody.

Quick Database Links

Gene Symbol

CHD8

UniProt

Q9HCK8

RefSeq (Protein):NP_065971.2

UniProt Details

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No UniProt data available

NCBI Reference Sequences

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Associated Accession Numbers

Curated reference sequences for the gene transcript and protein product

Protein	NP_065971.2

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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Immunoprecipitation

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