Rabbit DDX3 Antibody

SKU: orb1530142

Description

DDX3 Antibody

Research Area

Cancer Biology, Immunology & Inflammation

Images & Validation

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Item 1 of 4

Tested Applications	IHC, IP, WB
Dilution Range	WB - 1:1,000 - 1:10,000; IP - 2 - 10 µg/mg lysate; IHC - 1:200 to 1:1000. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
Reactivity	Human, Mouse
Application Notes	Format: Whole IgG

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	Between 1 and 50
Target	DDX3
Purification	Antigen Affinity Purified
Conjugation	Unconjugated

Storage & Handling

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Storage	2 - 8°C
Form/Appearance	Liquid
Buffer/Preservatives	Tris-buffered Saline containing 0.1% rAlbumin and 0.09% Sodium Azide
Concentration	200 µg/ml
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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ATP-dependent RNA helicase DDX3X; CAP-Rf; DBX; DDX14; DDX3; DEAD (Asp-Glu-Ala-Asp) box helicase 3, X-linked; DEAD (Asp-Glu-Ala-Asp) box polypeptide 3, X-linked; DEAD box protein 3, X-chromosomal; DEAD box, X isoform; DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 3; DEAD/H box-3; helicase-like protein 2; HLP2; MRX102

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Detection of human DDX3 by western blot.Samples: Whole cell lysate from NIH 3T3, CT26, CH27, TCMK-1, and BW5147.3 cells. Antibody: Affinity purified rabbit anti-DDX3 antibody at 0.04 µg/ml.

Detection of human DDX3 by western blot.Samples: Whole cell lysate from HeLa, HEK293T, MCF-7, Hep-G2, and Jurkat cells. Antibody: Affinity purified rabbit anti-DDX3 antibody orb1530142 at 0.04 µg/ml.

Detection of human DDX3 by immunohistochemistry. Sample: FFPE section of human testicular seminoma. Antibody: Affinity purified rabbit anti-DDX3 used at a dilution of 1:200 (1µg/ml).

Detection of human DDX3 by western blot of immunoprecipitates.Samples: Whole cell lysate from HEK293T cells. Antibodies: Affinity purified rabbit anti-DDX3 antibody.

Quick Database Links

Gene Symbol

DDX3

UniProt

O00571

RefSeq (Protein):NP_001347.2

UniProt Details

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No UniProt data available

NCBI Reference Sequences

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Associated Accession Numbers

Curated reference sequences for the gene transcript and protein product

Protein	NP_001347.2

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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Immunoprecipitation

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