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Rabbit DDX5 Antibody
SKU: orb1530059
Description
Research Area
Cancer Biology, Immunology & Inflammation
Images & Validation
−Item 1 of 5
| Tested Applications | IHC, IP, WB |
|---|---|
| Dilution Range | WB - 1:2,000 - 1:10,000; IP - 2 - 10 µg/mg lysate; IHC - 1:200 to 1:1000. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections. |
| Reactivity | Human, Mouse |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Between 564 and C-terminus |
| Target | DDX5 |
| Purification | Antigen Affinity Purified |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | 2 - 8°C |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | Tris-buffered Saline containing 0.1% rAlbumin and 0.09% Sodium Azide |
| Concentration | 200 µg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−ATP-dependent RNA helicase DDX5; DEAD (Asp-Glu-Ala-Asp) box helicase 5; DEAD (Asp-Glu-Ala-Asp) box polypeptide 5; DEAD box protein 5; DEAD box-5; DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 5 (RNA helicase, 68kD); G17P1; HLR1; HUMP68; p68; probable ATP-dependent RNA helicase DDX5; RNA helicase p68
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Detection of human DDX5 by western blot.Samples: Whole cell lysate from HeLa, HEK293T, and Jurkat cells. Antibody: Affinity purified rabbit anti-DDX5 antibody orb1530059 at 0.04 µg/ml.
Detection of mouse DDX5 by western blot.Samples: Whole cell lysate from TCMK-1 and NIH 3T3 cells. Antibody: Affinity purified rabbit anti-DDX5 antibody orb1530059 at 0.04 µg/ml.
Detection of mouse DDX5 by immunohistochemistry. Sample: FFPE section of mouse skin. Antibody: Affinity purified rabbit anti-DDX5 used at a dilution of 1:1000 (0.2µg/ml).
Detection of human DDX5 by immunohistochemistry. Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-DDX5 used at a dilution of 1:1000 (0.2µg/ml).
Detection of human DDX5 by western blot of immunoprecipitates.Samples: Whole cell lysate from HeLa cells. Antibodies: Affinity purified rabbit anti-DDX5 antibody.
Quick Database Links
UniProt Details
− No UniProt data available
NCBI Reference Sequences
−Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product| Protein | NP_004387.1 |
|---|
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Datasheet
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
IP
Immunoprecipitation
Rabbit DDX5 Antibody (orb1530059)
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