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Rabbit ETV3 Antibody
SKU: orb1524279
Description
Images & Validation
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| Tested Applications | IP, WB |
|---|---|
| Dilution Range | WB - 1:2,000 - 1:10,000; IP - 2 - 10 µg/mg lysate |
| Reactivity | Human |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Between 462 and C-terminus |
| Target | ETV3 |
| Purification | Antigen Affinity Purified |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | 2 - 8°C |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide |
| Concentration | 1000 µg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−ETS domain transcriptional repressor PE1; ETS translocation variant 3; ets variant gene 3, ETS family transcriptional repressor; METS; mitogenic Ets transcriptional suppressor; PE1; PE-1
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Detection of human ETV3 by western blot and immunoprecipitation. Samples: Whole cell lysate from Jurkat (15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) , HeLa (H; 50 µg) and HEK293T (T; 50 µg) cells. Antibodies: Affinity purified rabbit anti-ETV3 antibody orb1524279 used for WB at 0.1 µg/ml (A) and 1 µg/ml (B) and used for IP at 6 µg/mg lysate. ETV3 was also immunoprecipitated by rabbit anti-ETV3 antibody, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).
Quick Database Links
UniProt Details
− No UniProt data available
NCBI Reference Sequences
−Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product| Protein | NP_001138784.1 |
|---|
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Datasheet
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Protocol Information
WB
Western Blot (IB, immunoblot)
IP
Immunoprecipitation
Rabbit ETV3 Antibody (orb1524279)
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