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Rabbit GRLF1/p190RhoGAP Antibody
SKU: orb1527883
Description
Research Area
Cancer Biology, Cardiovascular Research, Immunology & Inflammation, Neuroscience
Images & Validation
−Item 1 of 2
| Tested Applications | IP, WB |
|---|---|
| Dilution Range | WB - 1:2,000 - 1:10,000; IP - 2 - 5 µg/mg lysate |
| Reactivity | Human, Mouse |
| Predicted Reactivity | Canine, Rat |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Between 1200 and 1250 |
| Target | GRLF1/p190RhoGAP |
| Purification | Antigen Affinity Purified |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | 2 - 8°C |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | Tris-buffered Saline containing 0.1% rAlbumin and 0.09% Sodium Azide |
| Concentration | 200 µg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−glucocorticoid receptor DNA-binding factor 1; glucocorticoid receptor repression factor 1; GRF-1; GRLF1; P190A; P190-A; p190ARhoGAP; p190RhoGAP; rho GAP p190A; rho GTPase-activating protein 35
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Detection of mouse GRLF1/p190RhoGAP by western blot. Samples: Whole cell lysate (50 µg) from CT26.WT, 4T1, and Renca cells. Antibodies: Affinity purified rabbit anti-GRLF1/p190RhoGAP antibody orb1527883 used for WB at 0.04 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Detection of human GRLF1/p190RhoGAP by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 µg) cells. Antibodies: Affinity purified rabbit anti-GRLF1/p190RhoGAP antibody orb1527883 used for WB at 0.04 µg/ml (A) and 1 µg/ml (B) and used for IP at 3 µg/mg lysate. GRLF1/p190RhoGAP was also immunoprecipitated by rabbit anti-GRLF1/p190RhoGAP antibody, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 10 seconds (A) and 3 seconds (B).
Quick Database Links
UniProt Details
− No UniProt data available
NCBI Reference Sequences
−Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product| RefSeq | EAW57452.1 |
|---|
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Datasheet
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Protocol Information
WB
Western Blot (IB, immunoblot)
IP
Immunoprecipitation
Rabbit GRLF1/p190RhoGAP Antibody (orb1527883)
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