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Rabbit anti-Hakai Antibody
SKU: orb1525681
Description
Images & Validation
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| Tested Applications | IP, WB |
|---|---|
| Dilution range | WB - 1:2,000 - 1:10,000; IP - 2 - 5 µg/mg lysate |
| Reactivity | Human, Mouse |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Immunogen | Between 441 and C-terminus |
| Target | Hakai |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | 2 - 8°C |
|---|---|
| Form/Appearance | Whole IgG |
| Buffer/Preservatives | Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide |
| Concentration | 1000 µg/ml |
| Disclaimer | For research use only |
Alternative Names
−RING-type E3 ubiquitin transferase Hakai; RNF188; RING finger protein 188; HAKAI; E-cadherin binding protein E7; c-Cbl-like protein 1; Cbl proto-oncogene, E3 ubiquitin protein ligase-like 1; Cbl proto-oncogene like 1, E3 ubiquitin protein ligase; Cas-Br-M (murine) ecotropic retroviral transforming sequence-like 1; casitas B-lineage lymphoma-transforming sequence-like protein 1; E3 ubiquitin-protein ligase Hakai
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Detection of human and mouse Hakai by western blot (h&m) and immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) , HEK293T (T; 50 µg) and mouse NIH 3T3 (M; 50 µg) cells. Antibodies: Affinity purified rabbit anti-Hakai antibody orb1525681 used for WB at 0.1 µg/ml (A) and 1 µg/ml (B) and used for IP at 3 µg/mg lysate. Hakai was also immunoprecipitated by rabbit anti-Hakai antibody, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 10 seconds (B).
Quick Database Links
UniProt Details
− No UniProt data available
NCBI Reference Sequences
−Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product| Protein | NP_079090.2 |
|---|
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Protocol Information
WB
Western Blot (IB, immunoblot)
IP
Immunoprecipitation
Rabbit anti-Hakai Antibody (orb1525681)
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