Rabbit HECTD1 Antibody

SKU: orb1525781

Description

HECTD1 Antibody

Research Area

Neuroscience

Images & Validation

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Item 1 of 1

Tested Applications	IP, WB
Dilution Range	WB - 1:2,000 - 1:10,000; IP - 2 - 5 µg/mg lysate
Reactivity	Human
Predicted Reactivity	Mouse
Application Notes	Format: Whole IgG

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	Between 1 and 50
Target	HECTD1
Purification	Antigen Affinity Purified
Conjugation	Unconjugated

Storage & Handling

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Storage	2 - 8°C
Form/Appearance	Liquid
Buffer/Preservatives	Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration	1000 µg/ml
Disclaimer	For research use only

Alternative Names

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E3 ligase for inhibin receptor; E3 ubiquitin-protein ligase HECTD1; EULIR; HECT domain containing E3 ubiquitin protein ligase 1; HECT domain-containing protein 1; HECT-type E3 ubiquitin transferase HECTD1

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Detection of human HECTD1 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 µg) cells. Antibodies: Affinity purified rabbit anti-HECTD1 antibody orb1525781 used for WB at 0.1 µg/ml (A) and 1 µg/ml (B) and used for IP at 3 µg/mg lysate. HECTD1 was also immunoprecipitated by rabbit anti-HECTD1 antibody, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A and B).

Quick Database Links

Gene Symbol

HECTD1

UniProt

Q9ULT8

RefSeq:AAP13073.1

UniProt Details

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No UniProt data available

NCBI Reference Sequences

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Associated Accession Numbers

Curated reference sequences for the gene transcript and protein product

RefSeq	AAP13073.1

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Protocol Information

Western Blot (IB, immunoblot)

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Immunoprecipitation

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