Rabbit Horse Serum proteins Antibody

SKU: orb22339

Description

Precipitating polyclonal rabbit antiserum to guinea pig serum proteins. In precipitating techniques as immunoelectrophoresis and radial immunodiffusion to identify the presence of IgA in cat milk or other body fluids or to determine its concentration. To prepare an immune adsorbent for the purification of cat IgA from serum or exocrine secretions. Antisera to IgA or sIgA do not discriminate between serum IgA (monomeric and dimeric) and higher molecular forms such as secretory IgA. This antiserum is not intended for use in non-precipitating antibody-binding or other highly sensitive assays. This does not exclude the use of the antiserum in more sensitive techniques if proper controls are included.

Images & Validation

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Tested Applications	DOT, IEP, IP
Reactivity	Equine
Application Notes	Inter-species cross-reactivity is a normal feature of antibodies to animal proteins since homolo- gous proteins of different species frequently share antigenic determinants. This antiserum has not been adsorbed for such cross-reactivity. Consequently it is not species-specific.IE: In immunoelectrophoresis use 2 μl serum or equivalent against 120 μl antiserum. In double radial immunodiffusion (Ouchterlony) use a rosette arrangement with 10 μl antiserum in 3 mm diameter center well and 2 μl serum samples (neat and serially diluted in 2 mm diameter peripheral wells. Read more...

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Delipidated, heat inactivated, lyophilized, stable whole antiserum. No preservative added. Total protein and IgG concentrations in the antiserum are comparable to those of pooled normal rabbit serum. No foreign proteins added.
Concentration	Total protein and IgG concentrations in the antiserum are comparable to those of pooled normal rabbit serum. No foreign proteins added.
Disclaimer	For research use only

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Protocol Information

Immunoprecipitation

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DOT

Dot Blot

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IEP

Immunoelectrophoresis

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