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Rabbit HSV Tag Antibody

SKU: orb1530838

Description

HSV Tag Antibody

Images & Validation

Tested ApplicationsELISA, ICC, IP, WB
Dilution RangeWB - 1:1,000 - 1:30,000; IP - 1 - 4 µg/mg lysate; ICC - 1:100 - 1:400; ELISA - 1:1,000 - 1:30,000; for coating plates 1:100 - 1:500
ReactivityVirus
Application Notes
Format: Whole IgG. Application Information: All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.

Key Properties

Antibody TypePrimary Antibody
HostRabbit
ClonalityPolyclonal
IsotypeIgG
TargetHSV Tag
PurificationAntigen Affinity Purified
ConjugationUnconjugated

Storage & Handling

Storage2 - 8°C
Form/AppearanceLiquid
Buffer/PreservativesPhosphate Buffered Saline (PBS) containing 0.09% Sodium Azide
Concentration1 mg/ml
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

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Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at support@biorbyt.com.

Rabbit HSV Tag Antibody

Detection of HSV-tagged protein by western blot.Samples: 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein. Antibodies: Affinity purified, rabbit anti-HSV antibody orb1530838 at 0.04 µg/ml (1:25, 000).

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Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
ICC
Immunocytochemistry
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol
IP
Immunoprecipitation
View Protocol

Rabbit HSV Tag Antibody (orb1530838)

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0.1 mg
$ 420.00
DispatchUsually dispatched within 5-10 working days
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