Rabbit IP3R1 Antibody

SKU: orb1527112

Description

IP3R1 Antibody

Research Area

Cancer Biology, Cardiovascular Research, Immunology & Inflammation, Metabolism Research, Neuroscience

Images & Validation

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Item 1 of 4

Tested Applications	IHC, IP, WB
Dilution range	WB - 1:2,000 - 1:10,000; IP - 2 - 5 µg/mg lysate; IHC - 1:100 - 1:500. Epitope retrieval with Tris-EDTA pH 9.0 is recommended for FFPE tissue sections.; IHC-IF - 1:50 - 1:250
Reactivity	Human, Mouse
Predicted Reactivity	Rat
Application Notes	Format: Whole IgG

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	Between 2708 and C-terminus
Target	IP3R1
Purification	Antigen Affinity Purified
Conjugation	Unconjugated

Storage & Handling

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Storage	2 - 8°C
Form/Appearance	Liquid
Buffer/Preservatives	Tris-buffered Saline containing 0.1% rAlbumin and 0.09% Sodium Azide
Concentration	200 µg/ml
Disclaimer	For research use only

Alternative Names

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ACV; CLA4; inositol 1,4,5-triphosphate receptor, type 1; inositol 1,4,5-trisphosphate receptor type 1; INSP3R1; IP3 receptor; IP3 receptor isoform 1; IP3R; IP3R 1; IP3R1; PPP1R94; protein phosphatase 1, regulatory subunit 94; SCA15; SCA16; SCA29; type 1 inositol 1,4,5-trisphosphate receptor; type 1 InsP3 receptor

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IP, WB
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Polyclonal
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Unconjugated
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Detection of human IP3R1 by western blot. Samples: Whole cell lysate (5, 15, and 50 µg) from HeLa cells. Antibody: Affinity purified rabbit anti-IP3R1 antibody orb1527112 used at 0.04 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Detection of human IP3R1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-IP3R1 antibody orb1527112 used for IP at 3 µg/mg lysate.

Detection of mouse IP3R1 by immunohistochemistry.Sample: FFPE section of mouse cerebellum. Antibody: Affinity purified rabbit anti-IP3R1 (Cat. No. orb1527112) used at a dilution of 1: 100 (2µg/ml). Detection: Red-fluorescent goat anti-rabbit IgG-heavy and light chain cross-adsorbed Antibody DyLight® 594 Conjugated used at a dilution of 1: 100. Counterstain: DAPI (blue).

Quick Database Links

Gene Symbol

IP3R1

UniProt

Q14643

RefSeq (Protein):NP_002213.5

UniProt Details

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No UniProt data available

NCBI Reference Sequences

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Associated Accession Numbers

Curated reference sequences for the gene transcript and protein product

Protein	NP_002213.5

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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Immunoprecipitation

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