Rabbit NF-kappaB2 Antibody

SKU: orb1527727

Description

NF-kappaB2 Antibody

Research Area

Cancer Biology, Immunology & Inflammation, Neuroscience

Images & Validation

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Item 1 of 2

Tested Applications	IP, WB
Dilution Range	WB - 1:2,000 - 1:10,000; IP - 2 - 10 µg/mg lysate
Reactivity	Human, Mouse
Application Notes	Format: Whole IgG

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	Between 850 and C-terminus
Target	NF-kappaB2 (p100)
Purification	Antigen Affinity Purified
Conjugation	Unconjugated

Storage & Handling

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Storage	2 - 8°C
Form/Appearance	Liquid
Buffer/Preservatives	Tris-buffered Saline containing 0.1% rAlbumin and 0.09% Sodium Azide
Concentration	200 µg/ml
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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CVID10; DNA-binding factor KBF2; H2TF1; lymphocyte translocation chromosome 10 protein; LYT10; LYT-10; NFKB, p52/p100 subunit; NF-kB2; nuclear factor Kappa-B, subunit 2; nuclear factor NF-kappa-B p100 subunit; nuclear factor NF-kappa-B p52 subunit; nuclear factor of Kappa light chain gene enhancer in B cells 2; Nuclear factor of kappa light polypeptide gene enhancer in B-cells 2; nuclear factor of kappa light polypeptide gene enhancer in B-cells 2 (p49/p100); oncogene Lyt-10; p100; p49/p100; p52; transcription factor NFKB2

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Detection of human and mouse NF-kappaB2 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-NF-kappaB2 antibody orb1527727 used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Detection of human NF-kappaB2 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-NF-kappaB2 antibody orb1527727 used for IP at 6 µg per reaction.

Quick Database Links

Gene Symbol

NF-kappaB2 (p100)

UniProt

Q00653

RefSeq (Protein):NP_002493.3

UniProt Details

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No UniProt data available

NCBI Reference Sequences

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Associated Accession Numbers

Curated reference sequences for the gene transcript and protein product

Protein	NP_002493.3

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Protocol Information

Western Blot (IB, immunoblot)

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Immunoprecipitation

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