Rabbit NFIC Antibody

SKU: orb1525420

Description

NFIC Antibody

Images & Validation

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Item 1 of 3

Tested Applications	IHC, IP, WB
Dilution Range	WB - 1:2,000 - 1:10,000; IP - 2 - 10 µg/mg lysate; IHC - 1:200 - 1:1,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Reactivity	Human, Mouse
Predicted Reactivity	Porcine
Application Notes	Format: Whole IgG

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	Between 200 and 250
Target	NFIC
Purification	Antigen Affinity Purified
Conjugation	Unconjugated

Storage & Handling

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Storage	2 - 8°C
Form/Appearance	Liquid
Buffer/Preservatives	Tris-buffered Saline containing 0.1% rAlbumin and 0.09% Sodium Azide
Concentration	200 µg/ml
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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CCAAT-box-binding transcription factor; CTF; CTF5; NF1-C; NFI; NF-I; NF-I/C; NFI-C; nuclear factor 1 C-type; Nuclear factor 1/C; Nuclear factor I/C; nuclear factor I/C (CCAAT-binding transcription factor); TGGCA-binding protein

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Detection of human NFIC by western blot and immunoprecipitation. Samples: Whole cell lysate (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-NFIC antibody orb1525420 used for WB at 0.04 µg/ml (A) and 1 µg/ml (B) and used for IP at 6 µg/mg lysate. NFIC was also immunoprecipitated by rabbit anti-NFIC antibody, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 3 seconds (B).

Detection of human NFIC by immunohistochemistry. Samples: FFPE serial sections of human breast carcinoma. Antibody: Affinity purified rabbit anti-NFIC Cat. No. orb1525420, left image used at a dilution of 1: 200 (1µg/ml) and Cat. No. orb1525418, right image used at a dilution of 1: 1, 000 (1µg/ml). Detection: DAB. Counterstain: Hematoxylin (blue).

Detection of mouse NFIC by immunohistochemistry. Samples: FFPE serial sections of mouse renal cell carcinoma. Antibody: Affinity purified rabbit anti-NFIC Cat. No. orb1525420, left image used at a dilution of 1: 200 (1µg/ml) and Cat. No. orb1525418, right image used at a dilution of 1: 1, 000 (1µg/ml). Detection: DAB

Quick Database Links

Gene Symbol

NFIC

UniProt

P08651

RefSeq:EAW69324.1

UniProt Details

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No UniProt data available

NCBI Reference Sequences

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Associated Accession Numbers

Curated reference sequences for the gene transcript and protein product

RefSeq	EAW69324.1

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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Immunoprecipitation

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