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Rabbit Notch2 Antibody
SKU: orb1527248
Description
Research Area
Cancer Biology, Cardiovascular Research, Immunology & Inflammation, Neuroscience
Images & Validation
−Item 1 of 3
| Tested Applications | FC, IHC, IP, WB |
|---|---|
| Dilution Range | WB - 1:2,000 - 1:10,000; IP - 2 - 5 µg/mg lysate; IHC - 1:100 - 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections. |
| Reactivity | Human |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Between 2421 and C-terminus |
| Target | Notch2 |
| Purification | Antigen Affinity Purified |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | 2 - 8°C |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | Tris-buffered Saline containing 0.1% rAlbumin and 0.09% Sodium Azide |
| Concentration | 200 µg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−AGS2; HJCYS; hN2; neurogenic locus notch homolog protein 2; Notch 2; Notch homolog 2
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Detection of human Notch2 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 µg) cells. Antibodies: Affinity purified rabbit anti-Notch2 antibody orb1527248 used for WB at 0.04 µg/ml (A) and 1 µg/ml (B) and used for IP at 3 µg/mg lysate. Notch2 was also immunoprecipitated by rabbit anti-Notch2 antibody, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).
Detection of human Notch2 by immunohistochemistry.Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-Notch2 (Cat. No. orb1527248) used at a dilution of 1: 200 (1µg/ml). Detection: DAB
Detection of human Notch2 (shaded) in U931 cells by flow cytometry.Antibody: Rabbit anti-Notch2 polyclonal antibody (orb1527248) or isotype control (unshaded). Secondary: DyLight® 488-conjugated goat anti-rabbit IgG.
Quick Database Links
UniProt Details
− No UniProt data available
NCBI Reference Sequences
−Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product| Protein | NP_077719.2 |
|---|
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Datasheet
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
FC
Flow Cytometry
IP
Immunoprecipitation
Rabbit Notch2 Antibody (orb1527248)
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