Rabbit ORP150/HYOU1 Antibody

SKU: orb1520223

Description

ORP150/HYOU1 Antibody

Images & Validation

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Item 1 of 2

Tested Applications	IP, WB
Dilution Range	WB - 1:10,000 - 1:25,000; IP - 20-40 µl/mg lysate
Reactivity	Human
Predicted Reactivity	Rat
Application Notes	Format: Whole IgG

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	Between 949 and C-terminus
Target	ORP150/HYOU1
Purification	Antigen Affinity Purified
Conjugation	Unconjugated

Storage & Handling

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Storage	2 - 8°C
Form/Appearance	Liquid
Buffer/Preservatives	Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration	1000 µg/ml
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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150 kDa oxygen-regulated protein; 170 kDa glucose-regulated protein; epididymis secretory sperm binding protein; Grp170; GRP-170; HSP12A; hypoxia up-regulated protein 1; ORP150; ORP-150; oxygen regulated protein (150kD)

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Detection of human ORP150/HYOU1 by western blot. Samples: Whole cell lysate (10 µg) from Hep-G2, MCF-7, U2OS, HeLa, and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-ORP150/HYOU1 antibody (orb1520223) used for WB at 0.04 mg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.

Detection of human ORP150/HYOU1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-ORP150/HYOU1 antibody (orb1520223) used for IP at 1 µg per reaction.

Quick Database Links

Gene Symbol

ORP150/HYOU1

UniProt

Q9Y4L1

RefSeq (Protein):NP_006380.1

UniProt Details

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No UniProt data available

NCBI Reference Sequences

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Associated Accession Numbers

Curated reference sequences for the gene transcript and protein product

Protein	NP_006380.1

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Protocol Information

Western Blot (IB, immunoblot)

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Immunoprecipitation

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