Rabbit Phospho MCM2 (S53) Antibody

SKU: orb1529687

Description

MCM2, Phospho (S53) Antibody

Images & Validation

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Item 1 of 2

Tested Applications	IHC, WB
Dilution Range	WB - 1:1,000 - 1:2,500; IHC - 1:100 - 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Reactivity	Human, Mouse
Application Notes	Format: Whole IgG

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	Surrounding serine 53
Target	MCM2 Phospho (S53)
Purification	Antigen Affinity Purified
Conjugation	Unconjugated

Storage & Handling

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Storage	2 - 8°C
Form/Appearance	Liquid
Buffer/Preservatives	Tris-buffered Saline containing 0.1% rAlbumin and 0.09% Sodium Azide
Concentration	200 µg/ml
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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BM28; CCNL1; cdc19; CDCL1; cell devision cycle-like 1; cyclin-like 1; D3S3194; DFNA70; DNA replication licensing factor MCM2; minichromosome maintenance deficient 2 (mitotin); minichromosome maintenance protein 2 homolog; MITOTIN; nuclear protein BM28

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Phospho-MCM2 (Ser53) Rabbit Polyclonal Antibody [orb185135]
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Phospho-MCM2 (Ser53) Rabbit Polyclonal Antibody (HRP) [orb503625]
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Phospho-MCM2 (Ser53) Rabbit Polyclonal Antibody (Biotin) [orb501719]
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Phospho-MCM2 (Ser53) Rabbit Polyclonal Antibody (AP) [orb939847]
Applications:
WB
Predicted Reactivity:
Canine, Equine, Gallus, Mouse, Porcine, Rabbit, Rat, Sheep, Zebrafish
Reactivity:
Human
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Rabbit
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Detection of human Phospho MCM2 (S53) by western blot.Samples: Whole cell lysate from asynchronous HEK293T cells. Antibody: Affinity purified rabbit anti-phospho MCM2 (S53) antibody orb1529687 used at 0.1 µg/ml.

Detection of human and mouse Phospho MCM2 (S53) by IHC. Sample: FFPE sections of human breast carcinoma (left) and mouse renal cell carcinoma (right). Antibody: Affinity purified rabbit anti-phospho MCM2 (S53) at a dilution of 1:200.

Quick Database Links

Gene Symbol

MCM2 Phospho (S53)

UniProt

P49736

RefSeq (Protein):NP_0045117.2

UniProt Details

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No UniProt data available

NCBI Reference Sequences

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Associated Accession Numbers

Curated reference sequences for the gene transcript and protein product

Protein	NP_0045117.2

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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