Rabbit Phospho RelA (S536) Antibody

SKU: orb1527302

Description

RelA, Phospho (S536) Antibody

Research Area

Cancer Biology, Cardiovascular Research, Immunology & Inflammation, Neuroscience

Images & Validation

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Item 1 of 2

Tested Applications	IHC, WB
Dilution Range	WB - 1:2,000 - 1:10,000; IP - Not recommended; IHC - 1:200 - 1:1,000. Epitope retrieval with Tris-EDTA pH 9.0 is recommended for FFPE tissue sections.
Reactivity	Human
Predicted Reactivity	Mouse
Application Notes	Format: Whole IgG

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	Surrounding serine 536
Target	RelA Phospho (S536)
Purification	Antigen Affinity Purified
Conjugation	Unconjugated

Storage & Handling

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Storage	2 - 8°C
Form/Appearance	Liquid
Buffer/Preservatives	Tris-buffered Saline containing 0.1% rAlbumin and 0.09% Sodium Azide
Concentration	200 µg/ml
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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NF-kappa-B p65delta3; NF-kappa-B transcription factor p65; NFKB3; nuclear factor NF-kappa-B p65 subunit; nuclear factor of kappa light polypeptide gene enhancer in B-cells 3; p65; transcription factor p65; v-rel avian reticuloendotheliosis viral oncogene homolog A; v-rel reticuloendotheliosis viral oncogene homolog A

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Detection of human phospho RelA-S536 by western blot. Samples: Whole cell lysate (15 and 50 µg) from Jurkat cells treated with TNF alpha and Calyculin A (+) or mock treated (-). Antibodies: Affinity purified rabbit anti-Phospho RelA (S536) antibody orb1527302 used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds. Lower panel: Western blot for total RelA using affinity purified rabbit anti-RelA antibody at 0.1 µg/ml with exposure time of 75 seconds.

Detection of human Phospho RelA (S536) by immunohistochemistry.Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-Phospho RelA (S536) (Cat. No. orb1527302) used at a dilution of 1: 200 (1µg/ml). Detection: DAB Counterstain: IHC Hematoxylin (blue).

Quick Database Links

Gene Symbol

RelA Phospho (S536)

UniProt

Q04206

RefSeq (Protein):NP_068810.3

UniProt Details

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No UniProt data available

NCBI Reference Sequences

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Associated Accession Numbers

Curated reference sequences for the gene transcript and protein product

Protein	NP_068810.3

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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