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Rabbit Phospho SMC1 (S966) Antibody
SKU: orb1530760
Description
Research Area
Immunology & Inflammation, Neuroscience
Images & Validation
−Item 1 of 5
| Tested Applications | ICC, IHC, IP, WB |
|---|---|
| Dilution Range | WB - 1:2,000 - 1:10,000; IP - 2 - 5 µg/mg lysate; IHC - 1:1,000 to 1:5,000. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.; ICC-IF - 1:500 - 1:5,000. Formaldehyde fixation is recommended. Permeabilization with Triton-X 100 is recommended for formaldehyde-fixed cells. |
| Reactivity | Human |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Surrounding serine 966 |
| Target | SMC1 Phospho (S966) |
| Purification | Antigen Affinity Purified |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | 2 - 8°C |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide |
| Concentration | 1000 µg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−CDLS2; DXS423E; epididymis secretory sperm binding protein; SB1.8; segregation of mitotic chromosomes 1; SMC protein 1A; SMC1; SMC1 (structural maintenance of chromosomes 1, yeast)-like 1; SMC-1A; SMC1alpha; SMC-1-alpha; SMC1L1; SMCB; structural maintenance of chromosomes protein 1A
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Detection of Phospho SMC1 (S966) by western blot.Samples: Jurkat cells treated with 100 µM etoposide (+) or mock treated (-). Antibodies: Affinity purified rabbit anti-Phospho SMC1 (S966) antibody orb1530760 used at 0.1 µg/ml.
Detection of human Phospho SMC1 (S966) by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-Phospho SMC1 (S966) used at a dilution of 1:1, 000 (1µg/ml).
Detection of human Phospho SMC1 (S966) by immunohistochemistry. Sample: FFPE section of human testicular seminoma. Antibody: Affinity purified rabbit anti-Phospho SMC1 (S966) used at a dilution of 1:1, 000 (1µg/ml).
Detection of human Phospho SMC1 (S966) by western blot of immunoprecipitates.Samples: Whole cell lysate from Jurkat cells treated with 100 µM etoposide (+) or mock treated (-). Antibodies: Rabbit anti-Phospho SMC1 (S966) antibody.
Detection of human Phospho SMC1 (S966) by ICC.Samples: HeLa cells. Antibody: Affinity purified rabbit anti-Phospho SMC1 (S966) used at a dilution of 1:5, 000. Detection: Red fluorescent Anti-rabbit IgG-DyLight® 594 conjugated.
Quick Database Links
UniProt Details
− No UniProt data available
NCBI Reference Sequences
−Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product| Protein | NP_006297.2 |
|---|
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Datasheet
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
ICC
Immunocytochemistry
IP
Immunoprecipitation
Rabbit Phospho SMC1 (S966) Antibody (orb1530760)
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