Rabbit Phospho ZAP70 (Y493) Antibody

SKU: orb1527395

Description

ZAP70, Phospho (Y493) Antibody

Research Area

Immunology & Inflammation, Neuroscience

Images & Validation

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Item 1 of 2

Tested Applications	IP
Dilution Range	WB - Not recommended. orb1527395 has not performed satisfactorily when used for WB of phospho ZAP70 in crude preparations (e.g. whole cell lysate). This antibody can be used for WB of enriched (e.g. immunoprecipitated) sources of ZAP70.; IP - 2 - 5 µg/mg lysate
Reactivity	Human
Predicted Reactivity	Mouse
Application Notes	Format: Whole IgG

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	Surrounding tyrosine 493
Target	ZAP70 Phospho (Y493)
Purification	Antigen Affinity Purified
Conjugation	Unconjugated

Storage & Handling

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Storage	2 - 8°C
Form/Appearance	Liquid
Buffer/Preservatives	Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration	1000 µg/ml
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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70 kDa zeta-associated protein; 70 kDa zeta-chain associated protein; ADMIO2; IMD48; SRK; STCD; STD; syk-related tyrosine kinase; tyrosine-protein kinase ZAP-70; TZK; ZAP-70; zeta chain of T cell receptor associated protein kinase 70kDa; zeta-chain (TCR) associated protein kinase 70kDa; zeta-chain associated protein kinase, 70kD

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Detection of Phosphorylation of human ZAP70 on Y319, Y493 or Y493/Y494 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from Jurkat cells that had been treated with hydrogen peroxide. Antibodies: Affinity purified rabbit anti-Phospho ZAP70 antibodies used for IP at 3 µg/mg lysate.

Detection of Phosphorylation of human ZAP70 on Y493 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from Jurkat cells that had been treated with hydrogen peroxide (+) or mock treated (-). Antibodies: Affinity purified rabbit anti-ZAP70 antibody was used at 3 µg/mg lysate to immunoprecipitate ZAP70. The immunoprecipitates were treated with phosphatase (+) or mock (-) treated.

Quick Database Links

Gene Symbol

ZAP70 Phospho (Y493)

UniProt

P43403

RefSeq (Protein):NP_001070.2

UniProt Details

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No UniProt data available

NCBI Reference Sequences

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Associated Accession Numbers

Curated reference sequences for the gene transcript and protein product

Protein	NP_001070.2

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Protocol Information

Immunoprecipitation

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