Rabbit PRPF8 Antibody

SKU: orb1523931

Description

PRPF8 Antibody

Images & Validation

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Item 1 of 3

Tested Applications	IHC, IP, WB
Dilution Range	WB - 1:2,000 - 1:10,000; IP - 2 - 10 µg/mg lysate; IHC - 1:500 - 1:2,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Reactivity	Human, Mouse
Application Notes	Format: Whole IgG

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	Between 1825 and 1875
Target	PRPF8
Purification	Antigen Affinity Purified
Conjugation	Unconjugated

Storage & Handling

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Storage	2 - 8°C
Form/Appearance	Liquid
Buffer/Preservatives	Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration	1000 µg/ml
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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220 kDa U5 snRNP-specific protein; apoptosis-regulated protein 1; apoptosis-regulated protein 2; HPRP8; p220; precursor mRNA processing protein; pre-mRNA-processing-splicing factor 8; PRP8; PRP8 homolog; PRP8 pre-mRNA processing factor 8 homolog; PRPC8; RP13; SNRNP220; splicing factor Prp8; U5 snRNP-specific protein (220 kD), ortholog of S. cerevisiae Prp8p

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Detection of human and mouse PRPF8 by western blot. Samples: Whole cell lysate from HEK293T (15 and 50 µg) , HeLa (50µg) , Jurkat (50µg) , and mouse NIH 3T3 (50µg) cells. Antibodies: Affinity purified rabbit anti-PRPF8 antibody orb1523931 used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Detection of human PRPF8 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HEK293T cells. Antibodies: Affinity purified rabbit anti-PRPF8 antibody orb1523931 used for IP at 6 µg/mg lysate.

Detection of human PRPF8 by immunohistochemistry. Samples: FFPE serial sections of human colon carcinoma. Antibody: Affinity purified rabbit anti-PRPF8 used at a dilution of 1: 1, 000 (1µg/ml). Detection: DAB

Quick Database Links

Gene Symbol

PRPF8

UniProt

Q6P2Q9

RefSeq (Protein):NP_006436.3

UniProt Details

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No UniProt data available

NCBI Reference Sequences

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Associated Accession Numbers

Curated reference sequences for the gene transcript and protein product

Protein	NP_006436.3

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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Immunoprecipitation

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