Rabbit RABGEF1 Antibody

SKU: orb1525755

Description

RABGEF1 Antibody

Images & Validation

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Item 1 of 2

Tested Applications	IHC, IP
Dilution Range	WB - Not recommended; IP - 2 - 5 µg/mg lysate; IHC - 1:1,000 - 1:5,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Reactivity	Human
Predicted Reactivity	Bovine, Mouse
Application Notes	Format: Whole IgG

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	Between 275 and 325
Target	RABGEF1
Purification	Antigen Affinity Purified
Conjugation	Unconjugated

Storage & Handling

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Storage	2 - 8°C
Form/Appearance	Liquid
Buffer/Preservatives	Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration	1000 µg/ml
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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RAB guanine nucleotide exchange factor (GEF) 1; rab5 GDP/GTP exchange factor; rabaptin-5-associated exchange factor for Rab5; RABEX5; rabex-5; RAP1

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Detection of human RABGEF1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-RABGEF1 antibody orb1525755 used for IP at 3 µg per reaction.

Detection of human RABGEF1 by immunohistochemistry.Sample: FFPE section of human lung cancer. Antibody: Affinity purified rabbit anti-RABGEF1 (Cat. No. orb1525755) used at a dilution of 1: 5, 000 (0.2µg/ml). Detection: DAB

Quick Database Links

Gene Symbol

RABGEF1

UniProt

Q9UJ41

RefSeq:BAC87138.1

UniProt Details

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No UniProt data available

NCBI Reference Sequences

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Associated Accession Numbers

Curated reference sequences for the gene transcript and protein product

RefSeq	BAC87138.1

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Protocol Information

IHC

Immunohistochemistry

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Immunoprecipitation

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