Rabbit RABGEF1 Antibody

SKU: orb1525757

Description

RABGEF1 Antibody

Images & Validation

−

Item 1 of 2

Tested Applications	IHC, IP, WB
Dilution range	WB - 1:2,000 - 1:10,000; IP - 2 - 5 µg/mg lysate; IHC - 1:500 - 1:2,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Reactivity	Human, Mouse
Predicted Reactivity	Bovine
Application Notes	Format: Whole IgG

Key Properties

−

Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	Between 25 and 75
Target	RABGEF1
Purification	Antigen Affinity Purified
Conjugation	Unconjugated

Storage & Handling

−

Storage	2 - 8°C
Form/Appearance	Liquid
Buffer/Preservatives	Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration	1000 µg/ml
Disclaimer	For research use only

Alternative Names

−

RAB guanine nucleotide exchange factor (GEF) 1; rab5 GDP/GTP exchange factor; rabaptin-5-associated exchange factor for Rab5; RABEX5; rabex-5; RAP1

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at support@biorbyt.com.

Detection of human and mouse RABGEF1 by western blot (h&m) and immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) , HEK293T (T; 50 µg) and mouse NIH 3T3 (M; 50 µg) cells. Antibodies: Affinity purified rabbit anti-RABGEF1 antibody orb1525757 used for WB at 0.1 µg/ml (A) and 1 µg/ml (B) and used for IP at 3 µg/mg lysate. RABGEF1 was also immunoprecipitated by rabbit anti-RABGEF1 antibody, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).

Detection of human RABGEF1 by immunohistochemistry.Sample: FFPE section of human lung carcinoma. Antibody: Affinity purified rabbit anti-RABGEF1 (Cat. No. orb1525757) used at a dilution of 1: 1, 000 (1µg/ml). Detection: DAB

Quick Database Links

Gene Symbol

RABGEF1

UniProt

Q9UJ41

RefSeq:BAC87138.1

UniProt Details

−

No UniProt data available

NCBI Reference Sequences

−

Associated Accession Numbers

Curated reference sequences for the gene transcript and protein product

RefSeq	BAC87138.1

Documents Download

Datasheet

Product Information

Download

Request a Document

Protocol Information

Western Blot (IB, immunoblot)

View Protocol

IHC

Immunohistochemistry

View Protocol

Immunoprecipitation

View Protocol

Find More Protocols