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Rabbit RNA Polymerase II, Phospho (S5) Antibody
SKU: orb1523051
Description
Research Area
Metabolism Research, Neuroscience
Images & Validation
−Item 1 of 2
| Tested Applications | IHC, IP, WB |
|---|---|
| Dilution Range | WB - 1:2,000 - 1:10,000; IP - 2 - 10 µg/mg lysate; IHC - 1:500 to 1:2,000. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections. |
| Reactivity | Human, Mouse |
| Predicted Reactivity | Drosophila |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Surrounding serine 5 |
| Target | Phospho RNA Polymerase II (S5) |
| Purification | Antigen Affinity Purified |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | 2 - 8°C |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | Tris-buffered Saline containing 0.1% rAlbumin and 0.09% Sodium Azide |
| Concentration | 200 µg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−DNA-directed RNA polymerase II largest subunit, RNA polymerase II 220 kd subunit; DNA-directed RNA polymerase II subunit A; DNA-directed RNA polymerase II subunit RPB1; DNA-directed RNA polymerase III largest subunit; hRPB220; hsRPB1; POLR2; POLRA; polymerase (RNA) II (DNA directed) polypeptide A, 220kDa; polymerase (RNA) II subunit A; RNA polymerase II subunit B1; RNA-directed RNA polymerase II subunit RPB1; RPB1; RPBh1; RpIILS; RPO2; RPOL2
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Detection of human Phospho RNA Polymerase II (S5) by western blot. Samples: Whole cell lysate (2 µg) from HeLa cells prepared using NETN lysis buffer. Lysates were either mock treated (-) or treated with phosphatases (+). Antibody: Affinity purified rabbit anti-Phospho RNA Polymerase II (S5) antibody (orb1523051) used for WB at 0.04 µg/ml. Detection: Chemiluminescence with an exposure time of 3 seconds. Lower Panel: Rabbit anti-RNA Polymerase II antibody.
Detection of human Phospho RNA Polymerase II (S5) by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 1% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Immunoprecipitates were either mock treated (-) or treated with phosphatases (+). Antibodies: Affinity purified rabbit anti-Phospho RNA Polymerase II (S5) antibody (orb1523051) used for IP at 6 µg per reaction.
Quick Database Links
UniProt Details
− No UniProt data available
NCBI Reference Sequences
−Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product| Protein | NP_000928.1 |
|---|
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Datasheet
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
IP
Immunoprecipitation
Rabbit RNA Polymerase II, Phospho (S5) Antibody (orb1523051)
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