Rabbit SF3A3 Antibody

SKU: orb1526498

Description

SF3A3 Antibody

Images & Validation

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Item 1 of 2

Tested Applications	IHC, IP, WB
Dilution range	WB - 1:2,000 - 1:10,000; IP - 2 - 5 µg/mg lysate; IHC - 1:100 - 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Reactivity	Human, Mouse
Application Notes	Format: Whole IgG

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	Between 451 and C-terminus
Target	SF3A3
Purification	Antigen Affinity Purified
Conjugation	Unconjugated

Storage & Handling

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Storage	2 - 8°C
Form/Appearance	Liquid
Buffer/Preservatives	Tris-buffered Saline containing 0.1% rAlbumin and 0.09% Sodium Azide
Concentration	200 µg/ml
Disclaimer	For research use only

Alternative Names

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pre-mRNA splicing factor SF3a (60kD); PRP9; PRPF9; SAP 61; SAP61; SF3a60; spliceosome associated protein 61; Spliceosome-associated protein 61; splicing factor 3A subunit 3; splicing factor 3a, subunit 3, 60kD; splicing factor 3a, subunit 3, 60kDa

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Detection of human and mouse SF3A3 by western blot (h&m) and immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) , HEK293T (T; 50 µg) , and mouse NIH 3T3 (M; 50 µg) cells. Antibodies: Affinity purified rabbit anti-SF3A3 antibody orb1526498 used for WB at 0.04 µg/ml (A) and 0.4 µg/ml (B) and used for IP at 3 µg/mg lysate. SF3A3 was also immunoprecipitated by rabbit anti-SF3A3 antibody, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 10 seconds (B).

Detection of human SF3A3 by immunohistochemistry.Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti-SF3A3 (Cat. No. orb1526498) used at a dilution of 1: 200 (1µg/ml). Detection: DAB

Quick Database Links

Gene Symbol

SF3A3

UniProt

Q12874

RefSeq (Protein):NP_006793.1

UniProt Details

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No UniProt data available

NCBI Reference Sequences

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Associated Accession Numbers

Curated reference sequences for the gene transcript and protein product

Protein	NP_006793.1

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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Immunoprecipitation

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